Anne Martin

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• Type de publi. : Article dans une revue
• Date de publi. : 01/09/2014
• Auteurs : Nathalie HolicAbabacar K. SeyeSaliha MajdoulSamia MartinOtto‐wilhelm MertenAnne GalyDavid Fenard
• Organismes : Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes Immunologie moléculaire et biothérapies innovantes
• Publié dans Human gene therapy. Clinical development le 28/10/2020

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Résumé : Abstract Human immunodeficiency virus type 1-derived lentiviral vectors (LVs) are becoming major tools for gene transfer approaches. Several gene therapy clinical studies involving LVs are currently ongoing. Industrial production of clinical-grade LVs is therefore an important challenge. Some improvements in LV production protocols have already been possible by acting on multiple steps of the production process like transfection, cell culture, or media optimizations. Yet, the effects of physicochemical parameters such as pH remain poorly studied. Mammalian cell cultures are generally performed at neutral pH, which may not be the optimal condition to produce high quantities of LVs with optimal infectious properties. In this study, we showed that lentiviral transient production in HEK293T cells is inversely dependent on the pH value of the harvesting medium. Infectious and physical titers of LVs pseudotyped with GALVTR or VSV-G glycoproteins are enhanced by two- to threefold at pH 6 compared with neutral conditions. pH 6-produced LVs are highly infectious on cell lines but also on relevant primary target cells like hCD34+ hematopoietic stem/progenitor cells. GALVTR-LV particles produced at pH 6 are highly stable at 37°C and resistant to multiple freeze-thaw cycles. Higher levels of expression of intracellular pr55gag polyproteins are observed within HEK293T producer cells cultured at pH 6. The positive effect of pH 6 conditions is also observed for moloney-derived retroviral vectors produced from NIH-3T3 fibroblasts, arguing that the mildly acidic pH effect is not limited to the lentivirus genus and is reproducible in various producer cell lines. This observation may help us in the design of more effective LV production protocols for clinical applications.

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Fichiers liés : Holic_et_al_HUMC_HAL.pdf

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• Type de publi. : Communication dans un congrès
• Date de publi. : 25/08/2014
• Auteurs : Anne LemoineSéverine BernardieOlivier BrivoisFlorent de MartinNicolas DesramautSylvestre Le RoyDaniel MonfortC. NegulescuRodrigo PedrerosOlivier SedanChan Quang VongAmélie VagnerEvelyne Foerster
• Organismes : Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières Bureau de Recherches Géologiques et Minières

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Résumé : The aim of the present study is to identify realistic and damaging scenarios of seismic and tsunami events on Nice city and to provide data relative to the resulting physical damages to the built environment, as well as to the network systems. We tried to reduce the gap between realistic scientific modeling, risk evaluation and mitigation. Regarding seismic risk scenario, very strong differences appear locally in the resulting damages, when using a finite fault 3D numerical model or empirical attenuation relationships. In this study, we show that refined earthquake modelling can improve risk assessment and therefore may be applied as input to evacuation models. However, spatial heterogeneities still need to be understood and strongly linked to ad hoc specifications (e. g. geological or seismotectonic context, source directivity, numerical methodology...) using detailed parametric analyses. As for the tsunami scenario considered in this study, very few consequences are to be expected in Nice city.

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• Type de publi. : Article dans une revue
• Date de publi. : 22/08/2014
• Auteurs : Etoungh MangaHugues BlascoPhilippe Da CostaMartin DrobekAndré AyralEmmanuel Le ClézioGilles DespauxBenoit CoasneA. Julbe
• Organismes : Institut Européen des membranes Institut Européen des membranes Institut Européen des membranes Institut d’Electronique et des Systèmes Matériaux, MicroCapteurs et Acoustique Institut d’Electronique et des Systèmes Matériaux, MicroCapteurs et Acoustique Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier Institut Européen des membranes
• Publié dans Langmuir le 27/10/2020

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• Type de publi. : Communication dans un congrès
• Date de publi. : 19/08/2014
• Auteurs : Bruno MartinIsabelle Verdier-MetzAnne FerlayJean-Louis BerdaguéAgnes CornuYves Y. ChilliardJean Baptiste J. B. CoulonMarie-Christine M.-C. Montel
• Organismes : Unité Mixte de Recherche sur les Herbivores - UMR 1213 Unité Mixte de Recherche Fromagère Unité Mixte de Recherche sur les Herbivores - UMR 1213 Qualité des Produits Animaux Unité Mixte de Recherche sur les Herbivores - UMR 1213 Unité Mixte de Recherche sur les Herbivores - UMR 1213 Unité Mixte de Recherche sur les Herbivores - UMR 1213 Unité Mixte de Recherche Fromagère

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Fichiers liés : Artisan_Cheese_2014_1.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 13/08/2014
• Auteurs : Jessica KastPatrizia HaneckerNathalie BeaufortArmin GieseAnne JoutelMartin DichgansChristian OpherkChristof Haffner
• Organismes : Institute for Stroke and Dementia Research Institute for Stroke and Dementia Research Institute for Stroke and Dementia Research Center for Neuropathology and Prion Research Génétique et Physiopathologie des Maladies Cérébro-Vasculaires Munich Center for Systems Neurology Institute for Stroke and Dementia Research Institute for Stroke and Dementia Research
• Publié dans Acta Neuropathologica Communications le 01/11/2020

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Résumé : Introduction:Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL) represents the most common hereditary form of cerebral small vessel disease characterized by early-onsetstroke and premature dementia. It is caused by mutations in the transmembrane receptor Notch3, which promote theaggregation and accumulation of the Notch3 extracellular domain (Notch3-ECD) within blood vessel walls. This processis believed to mediate the abnormal recruitment and dysregulation of additional factors including extracellular matrix(ECM) proteins resulting in brain vessel dysfunction. Based on recent evidence indicating a role for the transforminggrowth factor-β(TGF-β) pathway in sporadic and familial small vessel disease we studied fibronectin, fibrillin-1 andlatent TGF-βbinding protein 1 (LTBP-1), three ECM constituents involved in the regulation of TGF-βbioavailability, inpost-mortembrain tissue from CADASIL patients and control subjects.Results:Fibronectin and fibrillin-1 were found to be enriched in CADASIL vessels without co-localizing with Notch3-ECDdeposits, likely as a result of fibrotic processes secondary to aggregate formation. In contrast, LTBP-1 showed both anaccumulation and a striking co-localization with Notch3-ECD deposits suggesting specific recruitment into aggregates.We also detected increased levels of the TGF-βprodomain (also known as latency-associated peptide, LAP) indicatingdysregulation of the TGF-βpathway in CADASIL development.In vitroanalyses revealed a direct interaction betweenLTBP-1 and Notch3-ECD and demonstrated a specific co-aggregation of LTBP-1 with mutant Notch3.Conclusion:We propose LTBP-1 as a novel component of Notch3-ECD deposits and suggest its involvement inpathological processes triggered by Notch3-ECD aggregation.

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Fichiers liés : s40478-014-0096-8.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/08/2014
• Auteurs : Anne de Saint-MartinGabrielle RudolfCaroline SeegmullerMaria Paola Valenti-HirschEdouard Hirsch
• Organismes : Institut de génétique et biologie moléculaire et cellulaire Institut de génétique et biologie moléculaire et cellulaire Centre Hospitalier Universitaire [Strasbourg] Centre Hospitalier Universitaire [Strasbourg] Institut de génétique et biologie moléculaire et cellulaire
• Publié dans Epilepsia le 22/10/2020

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Résumé : Summary Epileptic encephalopathy with continuous diffuse spike‐waves during slow‐wave sleep ( ECSWS ) presents clinically with infrequent nocturnal focal seizures, atypical absences related to secondary bilateral synchrony, negative myoclonia, and atonic and rare generalized tonic–clonic seizures. The unique electroencephalography ( EEG) pattern found in ECSWS consists of continuous, diffuse, bilateral spike‐waves during slow‐wave sleep. Despite the eventual disappearance of clinical seizures and EEG abnormalities by adolescence, the prognosis is guarded in most cases because of neuropsychological and behavioral deficits. ECSWS has a heterogeneous etiology (genetic, structural, and unknown). Because epilepsy and electroencephalography (EEG) abnormalities in epileptic encephalopathy with continuous diffuse spike‐waves during slow‐wave sleep ( ECSWS ) are self‐limited and age related, the need for ongoing medical care and transition to adult care might be questioned. For adolescents in whom etiology remains unknown (possibly genetic) and who experience the disappearance of seizures and EEG abnormalities, there is rarely need for long‐term neurologic follow‐up, because often a relatively normal cognitive and social evolution follows. However, the majority of patients with structural and possibly “genetic syndromic” etiologies will have persistent cognitive deficits and will need suitable socioeducative care. Therefore, the transition process in ECSWS will depend mainly on etiology and its related features (epileptic active phase duration, and cognitive and behavioral evolution) and revolve around neuropsychological and social support rather than medical and pharmacologic follow‐up.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/08/2014
• Auteurs : Alejandro Niembro-MartinVictoria NasserddineEmmanuel PistonoHamza IssaAnne-Laure FrancTan-Phu Vuong
• Organismes : Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation Beirut Arab University [Beyrouth, Liban] Groupe de Recherche en Electromagnétisme Institut de Microélectronique, Electromagnétisme et Photonique - Laboratoire d'Hyperfréquences et Caractérisation
• Publié dans IEEE Transactions on Microwave Theory and Techniques le 31/10/2020

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Résumé : This paper describes a new concept of substrate integrated waveguide (SIW): a slow-wave substrate integrated waveguide (SW-SIW). Compared to a conventional SIW, the proposed topology requires a double-layer substrate with a bottom layer including internal metallized via-holes connected to the bottom conductive plane. The slow-wave effect is obtained by the physical separation of electric and magnetic fields in the structure. Electromagnetic simulations show that this topology of SIW allows decreasing the longitudinal dimension by more than 40% since the phase velocity is significantly smaller than that of a classical SIW. Simultaneously, the lateral dimension of the waveguide is also reduced. By considering a double-layer technology, SW-SIWs exhibiting a cutoff frequency of 9.3 GHz were designed, fabricated, and measured. The transversal dimension and the phase velocity of the proposed SW-SIW are both reduced by 40% as compared to a classical SIW designed for the same cutoff frequency, leading to a significant surface reduction. Moreover, an original kind of taper is proposed to achieve a good return loss when the SW-SIW is fed by a microstrip transmission line.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/08/2014
• Auteurs : Emeline Barbet-MassinMichele FellettiRobert SchneiderStefan JehleGuillaume CommunieNicolas MartinezMalene Ringkjøbing JensenRob W H RuigrokLyndon EmsleyAnne LesageMartin BlackledgeGuido Pintacuda
• Organismes : Biological Solid-State NMR Methods - Méthodes de RMN à l'état solide en biologie Institut des Sciences Analytiques Institut de biologie structurale Institut des Sciences Analytiques Institut de biologie structurale Laboratoire de virologie moléculaire et structurale Institut Laue-Langevin Institut de biologie structurale Unit of Virus Host Cell Interactions = Biologie structurale des interactions entre virus et cellule hôte Solid-State NMR Methods for Materials - Méthodes de RMN à l'état solide pour les matériaux Solid-State NMR Methods for Materials - Méthodes de RMN à l'état solide pour les matériaux Institut de biologie structurale Biological Solid-State NMR Methods - Méthodes de RMN à l'état solide en biologie
• Publié dans Biophysical Journal le 28/10/2020

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Résumé : (1)H-detected solid-state nuclear magnetic resonance (NMR) experiments are recorded on both intact and trypsin-cleaved sedimented measles virus (MeV) nucleocapsids under ultra-fast magic-angle spinning. High-resolution (1)H,(15)N-fingerprints allow probing the degree of molecular order and flexibility of individual capsid proteins, providing an exciting atomic-scale complement to electro microscopy (EM) studies of the same systems.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/08/2014
• Auteurs : Anne TréguierJ.M. PaillissonTony DejeanAlice ValentiniMartin A. SchlaepferJean-Marc Roussel
• Organismes : Écologie et santé des écosystèmes Ecosystèmes, biodiversité, évolution [Rennes] Ecosystèmes, biodiversité, évolution [Rennes] SPYGEN [Le Bourget-du-Lac] SPYGEN [Le Bourget-du-Lac] Écologie et santé des écosystèmes Écologie et santé des écosystèmes
• Publié dans Journal of Applied Ecology le 02/11/2020

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Résumé : 1. The introduction of non-native species is a major threat to biodiversity. While eradication programs of well-established invaders are costly and hazardous for non-target species, the early detection of a non-native species at low density is critical for preventing biological invasions in recipient ecosystems. Recent studies reveal that environmental DNA (eDNA) is a powerful tool for detecting target species in aquatic ecosystems, but these studies focus mostly on fish and amphibians.2. We examine the reliability of using eDNA to detect the presence of an invasive freshwater crustacean species, the red swamp crayfish Procambarus clarkii. Species-specific primers and probes were designed; their specificity was tested using in silico PCR simulations and against tissues of other crayfish species. Limits of detection and quantification were specified for the target DNA sequence by means of quantitative PCR amplifications on dilution series of known amount of P. clarkii DNA.3. The method was applied to water samples collected in 158 ponds in a French Nature Park, and results were compared to a traditional method using food-baited funnel traps. Environmental DNA had a better detection efficiency but predominantly led to divergent results compared with the trapping method. While habitat features partly explained the failure of crayfish detection by trapping, detection by eDNA was problematic at low crayfish abundances. When P. clarkii was detected, the estimated concentrations of crayfish DNA in water samples were always below the limit of quantification for the target DNA sequence.4. Synthesis and applications. The combination of environmental DNA (eDNA) and conventional trapping methods is recommended to monitor the invasion by P. clarkii in small waterbodies such as ponds. However, the risk of mortality for non-target species, notably amphibians, has to be carefully evaluated before large-scale deployment of traps. Contrary to fish and amphibians, a low amount of extracellular DNA in water is suspected to be the major limitation for crayfish detection by molecular approaches. Current advancements in PCR technology, together with optimization of the water sampling method, promise upcoming developments of eDNA detection for aquatic invertebrate species.

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• Type de publi. : Article dans une revue
• Date de publi. : 31/07/2014
• Auteurs : Solja T. NybergEleonor I FranssonKatriina HeikkiläKirsi AholaLars AlfredssonJakob B BjornerMarianne BorritzHermann BurrNico DraganoMarcel GoldbergMark HamerMarkus JokelaAnders KnutssonMarkku KoskenvuoAki KoskinenAnne KouvonenConstanze LeineweberIda E H MadsenLinda L Magnusson HansonMichael G MarmotMartin L NielsenMaria NordinTuula OksanenJan H PejtersenJaana PenttiReiner RuguliesPaula SaloJohannes SiegristAndrew SteptoeSakari SuominenTöres TheorellAri VäänänenJussi VahteraMarianna VirtanenPeter J M WesterholmHugo WesterlundMarie ZinsG David BattyEric J BrunnerJane E FerrieArchana Singh-ManouxMika Kivimäki
• Organismes : Finnish Institute of Occupational Health Institute of Environmental Medicine School of Health Sciences Finnish Institute of Occupational Health Finnish Institute of Occupational Health Institute of Environmental Medicine Centre for Occupational and Environmental Medicine National Research Centre for the Working Environment Department of Occupational and Environmental Medicine Federal Institute for Occupational Safety and Health Department of Medical Sociology Centre de recherche en épidémiologie et santé des populations Department of Epidemiology and Public Health Finnish Institute of Occupational Health and Institute of Behavioral Sciences Institute of Behavioural Sciences [Helsinki] Department of Health Sciences Department of Public Health Finnish Institute of Occupational Health School of Sociology, Social Policy, & Social Work UKCRC Centre of Excellence for Public Health (NI) Stress Research Institute National Research Centre for the Working Environment Stress Research Institute Department of Epidemiology and Public Health Department of Occupational and Environmental Medicine Department of Public Health Finnish Institute of Occupational Health The Danish National Centre for Social Research Finnish Institute of Occupational Health Department of Public Health [Copenhagen] National Research Centre for the Working Environment Finnish Institute of Occupational Health Department of Psychology Department of Medical Sociology Department of Epidemiology and Public Health Folkhälsan Research Center Department of Public Health Nordic School of Public Health Stress Research Institute Finnish Institute of Occupational Health Finnish Institute of Occupational Health Department of Public Health Finnisth Institute of Occupational Health Turku University Hospital Finnish Institute of Occupational Health Occupational and Environmental Medicine Stress Research Institute Centre de recherche en épidémiologie et santé des populations Department of Epidemiology and Public Health Centre for Cognitive Ageing and Cognitive Epidemiology Department of Epidemiology and Public Health Department of Epidemiology and Public Health School of Community and Social Medicine Centre de recherche en épidémiologie et santé des populations Department of Epidemiology and Public Health Finnish Institute of Occupational Health Department of Epidemiology and Public Health Department of Public Health [Helsinki]
• Publié dans Diabetes Care le 01/11/2020

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Résumé : The status of psychosocial stress at work as a risk factor for type 2 diabetes is unclear because existing evidence is based on small studies and is subject to confounding by lifestyle factors, such as obesity and physical inactivity. This collaborative study examined whether stress at work, defined as "job strain," is associated with incident type 2 diabetes independent of lifestyle factors. We extracted individual-level data for 124,808 diabetes-free adults from 13 European cohort studies participating in the IPD-Work Consortium. We measured job strain with baseline questionnaires. Incident type 2 diabetes at follow-up was ascertained using national health registers, clinical screening, and self-reports. We analyzed data for each study using Cox regression and pooled the study-specific estimates in fixed-effect meta-analyses. There were 3,703 cases of incident diabetes during a mean follow-up of 10.3 years. After adjustment for age, sex, and socioeconomic status (SES), the hazard ratio (HR) for job strain compared with no job strain was 1.15 (95% CI 1.06-1.25) with no difference between men and women (1.19 [1.06-1.34] and 1.13 [1.00-1.28], respectively). In stratified analyses, job strain was associated with an increased risk of diabetes among those with healthy and unhealthy lifestyle habits. In a multivariable model adjusted for age, sex, SES, and lifestyle habits, the HR was 1.11 (1.00-1.23). Findings from a large pan-European dataset suggest that job strain is a risk factor for type 2 diabetes in men and women independent of lifestyle factors.

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Fichiers liés : Nyberg et al. Job_strain_diabetes Diabetes Care.pdf

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