Philippe JEAN-PIERRE

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Jean-François CantaloubePierre GallianAmelia BokiloFrançois JordierPhilippe BiaginiHoussam AttouiChiaroni JacquesPhilippe de Micco
• Organismes : Anthropologie bio-culturelle, Droit, Ethique et Santé Anthropologie bio-culturelle, Droit, Ethique et Santé Anthropologie Bio-Culturelle CNTS of Brazzaville Anthropologie bio-culturelle, Droit, Ethique et Santé Anthropologie bio-culturelle, Droit, Ethique et Santé Institute for Animal Health Anthropologie bio-culturelle, Droit, Ethique et Santé Anthropologie bio-culturelle, Droit, Ethique et Santé
• Publié dans Journal of Medical Virology le 25/10/2020

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Résumé : The aim of this study was to assess the seroprevalence, viremia, genotype distribution, and demographic history of hepatitis C virus (HCV) in the Republic of the Congo. Testing was carried out on sera samples collected in 2005 from 807 Bantus belonging to the Kongo, Teke, and Ngala subgroups and 80 Pygmies. Positive HCV serology was found in 50 (5.6%) individuals including31 (60%) who were viremic. Seroprevalence increased with age with a cutoff at 50 years:2.8% <50 versus 12% >50. Twenty-one strains belonged to four described subtypes, that is, 4c in eight cases, 4h in two, 4k in three, and 4r in eight. Ten strains could not be assigned to any known subtype and may represent six new variants, that is, subtype 4 in five cases and subtype 2 in one. Evolutionary analysis of subtype 4c and 4r sequences indicated a period of enhanced transmission in the mid-twentieth century probably due to iatrogenic causes. This study underlines the high genetic diversity of strains in the Republic of the Congo with nine subtypes 4 and one subtype 2.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Fanny ChauxSlawomir FrynasHugo LaureanoChristine SalomonGérald MorataMarie-Laure AuclairMichel (massoud) StephanRachid MerdèsPhilippe RichardMarie-Jo Ondel-EyminJean-Christophe HenryJérôme BayardonChristophe DarcelSylvain Jugé
• Organismes : Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] SYNTHELOR SAS Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Biogéochimie et écologie des milieux continentaux Biogéochimie et écologie des milieux continentaux Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] SYNTHELOR SAS Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon] Organométalliques: Matériaux et Catalyse Institut de Chimie Moléculaire de l'Université de Bourgogne [Dijon]
• Publié dans Comptes Rendus. Chimie le 30/10/2020

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Résumé : Several approaches for the enantiodivergent synthesis of P-chirogenic mono- and diphosphines are described, using ephedrine methodology and phosphine borane chemistry. Firstly, both enantiomers of a tertiary phosphine can be obtained starting from the same oxazaphospholidine borane complex, prepared from (+)-ephedrine, when changing the order of addition of the organolithium reagents during the synthetic pathway. The second approach is based on the chlorophosphine boranes, which react with an organolithium reagent, to afford the corresponding phosphines with inversion of configuration. In the case where the chlorophosphine borane reacts with the t-butyl lithium reagent, a metal-halogen exchange occurs to afford the corresponding phosphide borane with retention of the configuration. The reaction of the phosphide borane with an alkyl halide leads to the same phosphine, but with the opposite configuration. Another approach depends on the diastereoselective preparation of the starting oxazaphospholidine borane complex from (−)-ephedrine, which leads according the case, to either one or the other enantiomer of a phosphine. Finally, the synthesis of (R,R)- and (S,S)-1,2-bis(methylphenylphosphino)ethane is also demonstrated using both enantiomers of the P-chirogenic diphosphinite diborane, which simultaneously allows the introduction of alkyl- or aryl substituents on the phosphorus atoms. In summary, these approaches show the great efficiency of the "ephedrine methodology" for the enantiodivergent synthesis of P-chirogenic mono- and diphosphines, and bearing alkyl or aryl substituents.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Natacha Entz-WerléPhilippe ChoquetAgnès NeuvilleSabine Kuchler-BoppFrançois ClaussJean-Marc DansePauline Simo-NoumbissieEric GuérinMarie-Pierre GaubJean-Noël FreundNelly BoehmAndré ConstantinescoPatrick LutzDominique GuenotFabienne Perrin-Schmitt
• Organismes : Service de Biophysique et Médecine Nucléaire Institut de Mécanique des Fluides et des Solides De l'homéostasie tissulaire au cancer et à l'inflammation Service de Biophysique et Médecine Nucléaire Service de Biophysique et Médecine Nucléaire Institut de Mécanique des Fluides et des Solides
• Publié dans Translational Oncology le 28/10/2020

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Résumé : TWIST and adenomatosis polyposis coli (APC) are critical signaling factors in normal bone development. In previous studies examining a homogeneously treated cohort of pediatric osteosarcoma patients, we reported the frequent and concurrent loss of both TWIST and APC genes. On these bases, we created a related animal model to further explore the oncogenic cooperation between these two genes. We performed intercrosses between twist-null/+ and Apc1638N/+ mice and studied their progeny. The Apc1638N/+; twist-null/+ mice developed bone abnormalities observed by macroscopic skeletal analyses and in vivo imaging. Complementary histologic, cellular, and molecular analyses were used to characterize the identified bone tumors, including cell culture and immunofluorescence of bone differentiation markers. Spontaneous localized malignant bone tumors were frequently identified in Apc1638N/+; twist-null/+ mice by in vivo imaging evaluation and histologic analyses. These tumors possessed several features similar to those observed in human localized osteosarcomas. In particular, the murine tumors presented with fibroblastic, chondroblastic, and osteoblastic osteosarcoma histologies, as well as mixtures of these subtypes. In addition, cellular analyses and bone differentiation markers detected by immunofluorescence on tumor sections reproduced most murine and human osteosarcoma characteristics. For example, the early bone differentiation marker Runx2, interacting physically with hypophosphorylated pRb, was undetectable in these murine osteosarcomas, whereas phosphorylated retinoblastoma was abundant in the osteoblastic and chondroblastic tumor subtypes. These characteristics, similar to those observed in human osteosarcomas, indicated that our animal model may be a powerful tool to further understand the development of localized osteosarcoma.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Philippe BonnardRoger SombiéFrançois-Xavier LescureAlain BougoumaJean-Baptiste Guiard-SchmidThierry PoynardPaul CalèsChantal HoussetPatrice CallardCatherine Le PendevenJoseph DraboFabrice CarratGilles Pialoux
• Organismes : AP-HP - Hôpital Tenon Service d’Hépato-gastroentérologie [Ouagadougou, Burkina-Faso] AP-HP - Hôpital Tenon Unité de Réseau d'Epidémiologie Clinique International Francophone Centre Hospitalier Universitaire Yalgado Ouédraogo [Ouagadougou, Burkina Faso] AP-HP - Hôpital Tenon AP-HP - Hôpital de la Pitié Salpêtrière Hémodynamique, Interaction Fibrose et Invasivité tumorales Hépatiques AP-HP - Hôpital de la Pitié Salpêtrière AP-HP - Hôpital Tenon Université Pierre et Marie Curie - Paris 6 AP-HP - Hôpital Tenon Centre Hospitalier Universitaire Yalgado Ouédraogo [Ouagadougou, Burkina Faso] Epidémiologie des maladies infectieuses et modélisation AP-HP - Hôpital Tenon
• Publié dans American Journal of Tropical Medicine and Hygiene le 25/10/2020

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Résumé :

AbstractLiver fibrosis (LF) must be assessed before talking treatment decisions in hepatitis B. In Burkina Faso, liver biopsy (LB) remains the “gold standard” method for this purpose. Access to treatment might be simpler if reliable alternative techniques for LF evaluation were available. The hepatitis B virus (HBV)-infected patients who underwent LB was invited to have liver stiffness measurement (Fibroscan) and serum marker assays. Fifty-nine patients were enrolled. The performance of each technique for distinguishing F0F1 from F2F3F4 was compared. The area under receiver operating characteristic (AUROC) curves was 0.61, 0.71, 0.79, 0.82, and 0.87 for the aspartate transaminase to platelet ratio index (APRI), Fib-4, Fibrotest, Fibrometre, and Fibroscan. Elastometric thresholds were identified for significant fibrosis and cirrhosis. Combined use of Fibroscan and a serum marker could avoid 80% of biopsies. This study shows that the results of alternative methods concord with those of histology in HBV-infected patients in Burkina Faso. These alternative techniques could help physicians to identify patients requiring treatment.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Naima G Cortes-PerezCatherine SapinLoïc JaffreloSabine DaouJean Pierre GrillPhilippe LangellaPhilippe SeksikLaurent BeaugerieSerge ChwetzoffGermain Trugnan
• Organismes : Université Pierre et Marie Curie - Paris 6 AP-HP - Hôpital Saint-Antoine Université Pierre et Marie Curie - Paris 6 AP-HP - Hôpital Saint-Antoine Université Pierre et Marie Curie - Paris 6 Université Pierre et Marie Curie - Paris 6 Trafic Membranaire et Signalisation Dans les Cellules Epitheliales
• Publié dans Journal of Biomedicine and Biotechnology le 24/10/2020

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Résumé : The delivery of bioactive molecules directly to damaged tissues represents a technological challenge. We propose here a new system based on virus-like particles (VLP) from rotavirus, with a marked tropism for the gut to deliver bio-active molecules to intestinal cells. For this, nonreplicative VLP nanoparticles were constructed using a baculovirus expression system and used to deliver an exogenous biomolecule, the green fluorescent protein (GFP), into either MA104 cells or intestinal cells from healthy and 2,4,6-trinitrobenzene sulfonic acid (TNBS)-treated mice. Our results show that expression of rotavirus capsid proteins in baculovirus led to the auto assembly of VLP that display similar properties to rotavirus. In vitro experiments showed that VLP were able to enter into MA104 cells and deliver the reporter protein. Intragastric administration of fluorescent VLP in healthy and TNBS-treated mice resulted in the detection of GFP and viral proteins in intestinal samples. Our results demonstrate an efficient entry of non-replicative rotavirus VLP into the epithelial cell line MA104 and provide the first in vivo evidence of the potential of these nanoparticles as a promising safe candidate for drug delivery to intestinal cells.

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Fichiers liés : 317545_1.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Romain PhilippeBrigitte CourtoisPierre MournetDenis FabreClaire BillotJean Christophe GlaszmannDominique ThisRedouane R. El-MalkiMarie-Christine Le PaslierDominique Brunel
• Organismes : Unité de Génétique Moléculaire Animale Développement et amélioration des plantes Développement et amélioration des plantes Développement et amélioration des plantes Développement et amélioration des plantes Développement et amélioration des plantes Développement et amélioration des plantes Etude du Polymorphisme des Génomes Végétaux Etude du Polymorphisme des Génomes Végétaux Etude du Polymorphisme des Génomes Végétaux
• Publié dans TAG Theoretical and Applied Genetics le 01/11/2020

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Résumé : Asr (ABA, stress, ripening) genes represent a small gene family potentially involved in drought tolerance in several plant species. To analyze their interest for rice breeding for water-limited environments, this gene family was characterized further. Genomic organization of the gene family reveals six members located on four different chromosomes and with the same exon-intron structure. The maintenance of six members of the Asr gene family, which are the result of combination between tandem duplication and whole genome duplication, and their differential regulation under water stress, involves probably some sub-functionalization. The polymorphism of four members was studied in a worldwide collection of 204 accessions of Oryza sativa L. and 14 accessions of wild relatives (O. rufipogon and O. nivara). The nucleotide diversity of the Asr genes was globally low, but contrasted for the different genes, leading to different shapes of haplotype networks. Statistical tests for neutrality were used and compared to their distribution in a set of 111 reference genes spread across the genome, derived from another published study. Asr3 diversity exhibited a pattern concordant with a balancing selection at the species level and with a directional selection in the tropical japonica sub-group. This study provides a thorough description of the organization of the Asr family, and the nucleotide and haplotype diversity of four Asr in Oryza sativa species. Asr3 stood out as the best potential candidate. The polymorphism detected here represents a first step towards an association study between genetic polymorphisms of this gene family and variation in drought tolerance traits

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Anouchka Guyon-DebastAlain LécureuilSandrine BonhommePhilippe GuercheJean-Luc Gallois
• Organismes : Institut Jean-Pierre Bourgin Institut Jean-Pierre Bourgin Institut Jean-Pierre Bourgin Institut Jean-Pierre Bourgin Génétique et Amélioration des Fruits et Légumes
• Publié dans BMC Plant Biology le 22/10/2020

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Résumé : Background: The proteasome subunit RPT5, which is essential for gametophyte development, is encoded by two genes in Arabidopsis thaliana; RPT5a and RPT5b. We showed previously that RPT5a and RPT5b are fully redundant in the Columbia (Col-0) accession, whereas in the Wassilewskia accession (Ws-4), RPT5b does not complement the effect of a strong rpt5a mutation in the male gametophyte, and only partially complements rpt5a mutation in the sporophyte. RPT5bCol-0 and RPT5bWs-4 differ by only two SNPs, one located in the promoter and the other in the seventh intron of the gene. Results: By exploiting natural variation at RPT5b we determined that the SNP located in RPT5b intron seven, rather than the promoter SNP, is the sole basis of this lack of redundancy. In Ws-4 this SNP is predicted to create a new splicing branchpoint sequence that induces a partial mis-splicing of the pre-mRNA, leading to the introduction of a Premature Termination Codon. We characterized 5 accessions carrying this A-to-T substitution in intron seven and observed a complete correlation between this SNP and both a 10 to 20% level of the RPT5b pre-mRNA missplicing and the lack of ability to complement an rpt5a mutant phenotype. Conclusion: The accession-dependent unequal redundancy between RPT5a and RPT5b genes illustrates an example of evolutionary drifting between duplicated genes through alternative splicing.

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Fichiers liés : 39147_20101228091359389_1.pdf

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• Type de publi. : Chapître d'ouvrage
• Date de publi. : 01/01/2010
• Auteurs : Yonghua Li-BeissonBasil ShorroshFred BeissonMats X. AnderssonVincent ArondelPhilippe D. BatesSébastien BaudDavid BirdAllan DebonoTimithy P. DurrettRochus B. FrankeIan A. GrahamKenta KatayamaAmelie A. KellyTony LarsonJonathan E. MarkhamMartine MiquelIsabel MolinaIkuo NishidaOwen RowlandLacey SamuelsKatherine M. SchmidHajime WadaRuth WeltiChangcheng XuRémi ZallotJohn Ohlrogge
• Organismes : Environnement, Bioénergie, Microalgues et Plantes Wirestone L.L.C. Environnement, Bioénergie, Microalgues et Plantes Department of Plant and Environmental Sciences Laboratoire de biogenèse membranaire Institute of Biological Chemistry Institut Jean-Pierre Bourgin Department of Chemical and Biological Sciences Independent Michigan State University [East Lansing] Rheinische Friedrich-Wilhelms-Universität Bonn Centre for Novel Agricultural Products, Department of Biology Independent University of Warwick [Coventry] Centre for Novel Agricultural Products, Department of Biology Donald Danforth Plant Science Center Institut Jean-Pierre Bourgin Algoma University Saitama University Department of Biology and Institute of Biochemistry Department of Botany Butler University The University of Tokyo Kansas Lipidomics Research Center, Division of Biology Brookhaven National Laboratory [Upton, NY] Laboratoire de biogenèse membranaire Independent

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Résumé : Lipides acyle dans Arabidopsis et toutes les autres plantes ont une myriade de fonctions diverses. Il s'agit notamment de fournir la barrière de diffusion de base des membranes qui sépare les cellules et les organites subcellulaires. Cette fonction implique seuls plus de 10 classes de lipides membranaires, y compris les phospholipides, galactolipides, et sphingolipides, et au sein de chaque classe, les variations dans la composition de la chaîne acyle augmenter le nombre de structures à plusieurs centaines de possibles espèces moléculaires. Lipides acyle dans le sous forme de compte triacylglycérol de 35% du poids de graines d'Arabidopsis et représentent leur forme majeure de carbone et de stockage d'énergie. Une couche de cutine et les cires cuticulaires qui limite la perte d'eau et offre une protection contre les invasions par des agents pathogènes et autres stress recouvre toute la surface aérienne d'Arabidopsis. Des fonctions similaires sont fournis par la subérine et des cires associés qui sont localisées dans les racines, les téguments et les zones d'abscission et sont produites en réponse à une blessure. Ce chapitre se concentre sur les voies métaboliques qui sont associés à la biosynthèse et la dégradation des lipides acyle mentionnés ci-dessus. Ces voies, les enzymes et les gènes sont également présentés en détail dans un site Web associé (ARALIP: http://aralip.plantbiology.msu.edu/ ). Protocoles et méthodes utilisés pour l'analyse des lipides Arabidopsis sont fournis. Enfin, un résumé détaillé de la composition des lipides Arabidopsis est fourni en trois chiffres et 15 tables.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Philippe RicaudBenjamin GabardSolène DerrienJean-Pierre ChaboureauThomas RoseAndreas MombauerHarald Czekala
• Organismes : Laboratoire d'aérologie Laboratoire d'aérologie Centre de Recherches Atmosphériques Laboratoire d'aérologie Fraunhofer Institute for Applied Information Technology RPG Radiometer Physics GmbH
• Publié dans IEEE Transactions on Geoscience and Remote Sensing le 31/10/2020

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Résumé : The H2O Antarctica Microwave Stratospheric and Tropospheric Radiometers (HAMSTRAD) program aims to develop two ground-based microwave radiometers to sound tropospheric and stratospheric water vapor (H2O) above Dome C (Concordia Station), Antarctica (75??06' S, 123??21'E, 3233 m asml), an extremely cold and dry environment, over decades. By using state-of-the-art technology, the HAMSTRAD-Tropo radiometer uses spectral information in the domains 51-59 GHz (oxygen line) and 169-197 GHz (water vapor line) to derive accurate tropospheric profiles of temperature (with accuracy ranging from 1 to 2 K) and low absolute humidity (with accuracy ranging from 0.02 to 0.05 g ?? m-3), together with integrated water vapor (with accuracy of about 0.008 kg ?? m-2) and liquid water path. Prior to its installation at Dome C in January 2009, the fully automated radiometer has been deployed at the Pic du Midi (PdM, 42??56'N, 0??08'E, 2877 m asml, France) in February 2008 and was in operation for five months. Preliminary comparisons with radio soundings particularly launched in the vicinity of PdM in February 2008 and the outputs from the mesoscale MESO-NH model show a great consistency to within 0.2-0.3 g ?? m-3 between all absolute humidity data sets whatever the atmosphere considered (extremely dry or wet).

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Fichiers liés : Ricaud2010.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2010
• Auteurs : Sébastien BoucksomPhilippe EyssidieuxVincent GuedjAhmed Zeriahi
• Organismes : Institut de Mathématiques de Jussieu Institut Fourier Institut de Mathématiques de Toulouse UMR5219 Institut de Mathématiques de Toulouse UMR5219
• Publié dans Acta Mathematica le 25/10/2020

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