Philippe JEAN-PIERRE

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• Type de publi. : Article dans une revue
• Date de publi. : 01/05/2008
• Auteurs : Min Ji KimClaude JardelCyrille BarthélémyVéronique JanJean Philippe BastardSandrine Fillaut-ChapinSydney HouryJacqueline CapeauAnne Lombès
• Organismes : Pathologie de l'Adipocyte et des Cellules Hepatiques Physiopathologie et thérapie du muscle strié
• Publié dans Antimicrobial Agents and Chemotherapy le 26/10/2020

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Résumé : ABSTRACT Lipoatrophy is a prevalent side effect of antiretroviral treatment of human immunodeficiency virus (HIV) infection. Its mechanisms are still disputed but include mitochondrial toxicity and, in particular, mitochondrial DNA (mtDNA) depletion induced by nucleoside reverse transcriptase inhibitors. To obtain an integrated evaluation of the mitochondrial alteration in lipoatrophy, we investigated the DNA, RNA, and protein levels in 15 samples of abdominal subcutaneous adipose tissue from HIV-infected patients with peripheral lipoatrophy and compared the results with those for 15 samples from age- and body mass index-matched controls. The DNA and RNA analyses used PCR-based techniques, while proteins were quantified with enzyme-linked immunosorbent assay and measurement of activities with spectrophotometric assays. Depletion of mtDNA and mtDNA-encoded MT-CO2 mRNA was present, but normal levels of mtDNA-dependent activity (cytochrome c oxidase) and protein (MT-CO2p) showed that it was compensated for. An increase in nuclear-DNA-dependent mitochondrial activities (citrate synthase and malate dehydrogenase) and protein (COX4I1p), as well as transcriptional up-regulation of nuclear-DNA-encoded mitochondrial genes ( COX4I1 and UCP2 ), demonstrated increased mitochondrial biogenesis. However, the expression of the known transcription factors of mitochondrial biogenesis ( TFAM , NRF1 , GABPA , PPARGC1A , PPARGC1B , and PPRC1 ) was normal or decreased. Increased amounts of activated caspase 3 and of DDIT3 mRNA showed the induction of apoptosis and oxidative stress, respectively. The mtDNA content did not correlate with any other mitochondrial parameter. In conclusion, mtDNA content does not appear to be an accurate biomarker of mitochondrial alteration in lipoatrophic adipose tissue. The preservation of mtDNA-dependent mitochondrial functions occurred despite severe mtDNA depletion. The presence of significant oxidative stress and apoptosis did not correlate with the mtDNA content.

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• Type de publi. : Article dans une revue
• Date de publi. : 30/04/2008
• Auteurs : Vincent VerbiestDanièle MontaudonMichel TautuJoyce MoukarzelJean-Pierre PortailJudith MarkovitsJacques RobertFrançois IchasPhilippe Pourquier
• Organismes : Laboratoire de Biologie et de Pharmacologie Appliquée Laboratoire Aimé Cotton Plateforme de génétique moléculaire des cancers d'Aquitaine Signalisation et Mecanismes Moleculaires de l'Apoptose
• Publié dans FEBS Letters le 24/10/2020

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Résumé : PRMT7 belongs to the protein arginine methyl-transferases family. We show that downregulation of PRMT7alpha and beta isoforms in DC-3F hamster cells was associated with increased sensitivity to the Top1 inhibitor camptothecin (CPT). This effect was not due to a change in Top1 contents or catalytic activity, or to a difference in the reversal of DNA breaks. Overexpression of PRMT7alpha and beta in DC-3F cells had no effect on CPT sensitivity, whereas it conferred a resistance to DC-3F/9-OH-E cells for which both isoforms are reduced by two- to three-fold as compared to DC-3F parental cells. Finally, downregulation of the human PRMT7 could also sensitize HeLa cells to CPT, suggesting that it could be used as a target to potentiate CPT derivatives.

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• Type de publi. : Article dans une revue
• Date de publi. : 28/04/2008
• Auteurs : Elise BonvinPhilippe Le RouzicJean-François BernaudinCharles-Henry CottartClarisse VandebrouckAntoine CrieTeresinha LealAnnick ClémentMonique Bonora
• Organismes : Physiologie, biologie des organismes, populations, interactions Physiologie, biologie des organismes, populations, interactions CHU Tenon [AP-HP] Faculté de Pharmacie de Paris - Université Paris Descartes Institut de physiologie et biologie cellulaires [UMR 6187] Université Pierre et Marie Curie - Paris 6 Université Catholique de Louvain = Catholic University of Louvain Physiologie, biologie des organismes, populations, interactions CHU Trousseau [APHP] Physiologie, biologie des organismes, populations, interactions
• Publié dans The Journal of Physiology le 29/10/2020

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Résumé : In cystic fibrosis (CF) patients, the major alteration in pulmonary function is due to peripheral airway obstruction. In the present study, we investigated the possibility that alterations in the extrathoracic airways, particularly in the trachea that expresses high levels of CFTR (CF transmembrane conductance regulator), may contribute to respiratory dysfunction. We performed morphological analyses of the trachea and airway functional studies in adult Cftr knockout (Cftr−/−) and F508del-CFTR mice and their controls. Macroscopic and histological examination of the trachea showed the presence of one to seven disrupted or incomplete cartilage rings in Cftr−/− mice (23/25) while only a few Cftr+/+ mice (6/25) had one abnormal ring. Tracheal defects were mainly localized in the proximal trachea. In 14 Cftr−/− mice, frontal disruption of the first three to six rings below the cricoid cartilage were associated with upper tracheal constriction. Similar tracheal abnormalities were detected in adult F508del-CFTR and in newborn Cftr−/− and F508del-CFTR mice. Tracheal and ventilatory function analyses showed in Cftr−/− mice a decreased contractile response of the proximal trachea and a reduced breathing rate due to an increase in the inspiratory and expiratory times. In F508del-CFTR mice, the expiratory time was longer than in controls. Therefore, these structural and functional abnormalities detected in adult and newborn CF mouse models may represent congenital malformations related toCFTRdysfunction. These results raise important questions concerning themechanismsgoverning trachealdevelopmentwithin the context ofCFTRproteindysfunction and the implication of such abnormalities in the pathogenesis of airway disease in CF

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• Type de publi. : Article dans une revue
• Date de publi. : 28/04/2008
• Auteurs : Edouard FerrandPatricia JabreClaire Vincent-GenodRégis AubryMichel BadetPhilippe BadiaAlain CariouFrançoise EllienValérie GounantRoger GilSamir JaberSylvie JayElena PaillaudPhilippe PoulainBernard RegnierJean ReignierGérard SociéBernard TardyFrançois LemaireChristian Brun-BuissonJean MartyNon Renseigné
• Organismes : Institut de Mathématiques de Jussieu Laboratoire d'Informatique pour l'Entreprise et les Systèmes de Production General ICU Réseau SPES Service d'anesthésie et de réanimation B Bioinformatique génomique et moléculaire Service de réanimation médicale Unité d'hématologie et de transplantation Groupe de recherche sur la thrombose, pharmacologie des antithrombotiques et situations à risque Physiopathologie et Thérapeutiques Respiratoires Service de réanimation médicale DAM Île-de-France
• Publié dans Archives of Internal Medicine le 30/07/2013

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Résumé : BACKGROUND: In developed countries at present, death mostly occurs in hospitals, but the circumstances and factors associated with the quality of organization and care surrounding death are not well described. METHODS: We designed a large multicenter cross-sectional study to analyze the setting and clinical course of each patient on the day of death. We included 2750 clinical departments of 294 hospitals. Of these, 1033 departments (37.6%) of 200 hospitals (68.0%) contributed to the Mort-a-l'Hôpital survey. Data were collected prospectively by the bedside nurse of each patient within 10 days of the occurrence of death. Main outcome measures included circumstances of death in hospitalized patients; secondary outcomes, nurses' perceptions of quality of end-of-life care. RESULTS: Of the 1033 participating departments, 420 recorded no deaths during the study period and 613 declared at least 1 death. In the 3793 patients who died and were included for assessment, only 925 (24.4%) had loved ones present at the time of death; 70.1% had respiratory distress during the period before death; and only 12.0% were in pain. Written protocols for end-of-life care were available in 12.2% of participating departments. Only 35.1% of nurses judged the quality of dying and death acceptable for themselves. Principal factors significantly associated with this perception were availability of a written protocol for end-of-life care, anticipation of death, informing the family, surrogate designation, adequate control of pain, presence of family or friends at the time of death, and staff meeting with the family after the death. CONCLUSIONS: This large prospective study identifies nonoptimal circumstances of death for hospitalized patients and a number of suggestions for improvement. A combination of factors reflected in the nurses' satisfaction may improve the quality of end-of-life care.

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• Type de publi. : Communication dans un congrès
• Date de publi. : 25/04/2008
• Auteurs : Danièle Valdés-LaoAndré CicolellaOlivier BlanchardLuc MosqueronJulien CaudevilleJean-Baptiste MassonAlain TrugeonChristophe DeclercqIsabelle GremyPhilippe PepinAbdoul SonkoOlivier Guye
• Organismes : Institut National de l'Environnement Industriel et des Risques Structure et fonctionnement des systèmes hydriques continentaux Institut National de l'Environnement Industriel et des Risques Institut National de l'Environnement Industriel et des Risques Institut National de l'Environnement Industriel et des Risques Institut National de l'Environnement Industriel et des Risques Institut National de l'Environnement Industriel et des Risques Observatoire régional de la santé Observatoire régional de la santé Observatoire régional de la santé Observatoire régional de la santé Observatoire régional de la santé Observatoire régional de la santé

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• Type de publi. : Communication dans un congrès
• Date de publi. : 23/04/2008
• Auteurs : Maëlenn GuerchetMoussiliou Noël ParaïsoPhilippe NubukpoDismand Stephan HouinatoJean-Pierre ClémentJean François DartiguesPierre-Marie Preux
• Organismes : Neuroépidémiologie Tropicale et Comparée Neuroépidémiologie Tropicale et Comparée Centre de Mémoire de Ressources et de Recherches [Limoges] Hôpital Psychiatrique La Valette Neuroépidémiologie Tropicale et Comparée Neuroépidémiologie Tropicale et Comparée Centre de Mémoire de Ressources et de Recherches [Limoges] Service de Psychiatrie [CHU Limoges] Neuroépidémiologie Tropicale et Comparée Epidémiologie et Biostatistique [Bordeaux] Neuroépidémiologie Tropicale et Comparée Laboratoire de Biostatistique et d'Informatique Médicale Service de l'Information Médicale et de l'Évaluation [CHU Limoges]

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• Type de publi. : Article dans une revue
• Date de publi. : 22/04/2008
• Auteurs : Camille BrochierMarie-Claude GaillardElsa DiguetNicolas CaudyCarole DossatBéatrice SegurensPatrick WinckerEmmanuel Flamand-RozeJocelyne CabochePhilippe HantrayeEmmanuel BrouilletJean-Marc ElaloufMichel de Chaldée
• Organismes : Service de Biologie Intégrative et Génétique Moléculaire Service de Biologie Intégrative et Génétique Moléculaire Institut d'Imagerie BioMédicale Service de Biologie Intégrative et Génétique Moléculaire Genoscope - Centre national de séquençage [Evry] Genoscope - Centre national de séquençage [Evry] Genoscope - Centre national de séquençage [Evry] Neurobiologie des processus adaptatifs Neurobiologie des processus adaptatifs Institut d'Imagerie BioMédicale Institut d'Imagerie BioMédicale Service de Biologie Intégrative et Génétique Moléculaire Service de Biologie Intégrative et Génétique Moléculaire
• Publié dans Physiological Genomics le 02/11/2020

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Résumé : Using serial analysis of gene expression, we collected quantitative transcriptome data in 11 regions of the adult wild-type mouse brain: the orbital, prelimbic, cingulate, motor, somatosensory, and entorhinal cortices, the caudate-putamen, the nucleus accumbens, the thalamus, the substantia nigra, and the ventral tegmental area. With >1.2 million cDNA tags sequenced, this database is a powerful resource to explore brain functions and disorders. As an illustration, we performed interregional comparisons and found 315 differential transcripts. Most of them are poorly characterized and 20% lack functional annotation. For 78 differential transcripts, we provide independent expression level measurements in mouse brain regions by real-time quantitative RT-PCR. We also show examples where we used in situ hybridization to achieve infrastructural resolution. For 30 transcripts, we next demonstrated that regional enrichment is conserved in the human brain. We then quantified the expression levels of region-enriched transcripts in the R6/2 mouse model of Huntington disease and the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease and observed significant alterations in the striatum, cerebral cortex, thalamus and substantia nigra of R6/2 mice and in the striatum of MPTP-treated mice. These results show that the gene expression data provided here for the mouse brain can be used to explore pathophysiological models and disclose transcripts differentially expressed in human brain regions.

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• Type de publi. : Communication dans un congrès
• Date de publi. : 21/04/2008
• Auteurs : Philippe CambierPierre BenoitGuillaume BodineauJean-Noel RamponAnne JaulinAmelie TrouveAnnie GuérinNicolas ProixM. Le Villio-PoitrenaudSabine Houot
• Organismes : Environnement et Grandes Cultures Environnement et Grandes Cultures Environnement et Grandes Cultures Environnement et Grandes Cultures Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés Laboratoire d'Analyses des Sols Laboratoire d'Analyses des Sols Centre de recherche sur la Propreté Environnement et Grandes Cultures

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Résumé : absent

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• Type de publi. : Communication dans un congrès
• Date de publi. : 21/04/2008
• Auteurs : Philippe CambierPierre BenoitGuillaume BodineauJean-Noel RamponAnne JaulinAmelie TrouveAnne GuerinNicolas ProixMaelenn Le Villio-PoitrenaudSabine Houot
• Organismes : Environnement et Grandes Cultures Environnement et Grandes Cultures Unité Expérimentale d'Amélioration des Arbres Forestiers Environnement et Grandes Cultures Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés Physicochimie et Ecotoxicologie des SolS d'Agrosystèmes Contaminés Unité de recherche Plantes et Systèmes de Culture Horticoles Laboratoire d'Analyses des Sols Veolia Environnement - Centre de recherche sur la Propreté, Limay, France Environnement et Grandes Cultures

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Résumé : absent

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• Type de publi. : Communication dans un congrès
• Date de publi. : 21/04/2008
• Auteurs : Laure MichelYves MéheustJ. BouquainJean-Pierre CaudalJean de Bremond d'ArsJean-Raynald de DreuzyPhilippe Davy
• Organismes : Géosciences Rennes Géosciences Rennes Géosciences Rennes Géosciences Rennes Géosciences Rennes Géosciences Rennes Géosciences Rennes

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