Philippe JEAN-PIERRE

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• Type de publi. : Article dans une revue
• Date de publi. : 06/06/2006
• Auteurs : Nadia JesselMustapha Oulad-AbdelghaniFlorent MeyerPhilippe LavalleYoussef HaïkelPierre SchaafJean-Claude Voegel
• Organismes : Biomatériaux : Processus biophysiques et biologiques aux interfaces Faculté de chirurgie dentaire - Robert Frank [Strasbourg] Institut de génétique et biologie moléculaire et cellulaire Institut Clinique de la Souris Biomatériaux : Processus biophysiques et biologiques aux interfaces Faculté de chirurgie dentaire - Robert Frank [Strasbourg] Biomatériaux : Processus biophysiques et biologiques aux interfaces Faculté de chirurgie dentaire - Robert Frank [Strasbourg] Biomatériaux : Processus biophysiques et biologiques aux interfaces Faculté de chirurgie dentaire - Robert Frank [Strasbourg] Institut Charles Sadron Biomatériaux : Processus biophysiques et biologiques aux interfaces Faculté de chirurgie dentaire - Robert Frank [Strasbourg]
• Publié dans Proceedings of the National Academy of Sciences of the United States of America le 31/10/2020

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Résumé : The basic premise of gene therapy is that genes can be used to produce in situ therapeutic proteins. The controlled delivery of DNA complexes from biomaterials offers the potential to enhance gene transfer by maintaining an elevated concentration of DNA within the cellular microenvironment. Immobilization of the DNA to the substrate to which cells adhere maintains the DNA in the cell microenvironment for subsequent cellular internalization. Here, layer-by-layer (LBL) films made from poly(L-glutamic acid) (PLGA) and poly(L-lysine) (PLL) containing DNA were built in the presence of charged cyclodextrins. The biological activities of these polyelectrolyte films were tested by means of induced production of a specific protein in the nucleus or in the cytoplasm by cells in contact with the films. This type of coating offers the possibility for either simultaneous or sequential interfacial delivery of different DNA molecules aimed at cell transfection. These results open the route to numerous potential applications in patch vaccination, for example.

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• Type de publi. : Communication dans un congrès
• Date de publi. : 05/06/2006
• Auteurs : Philippe LeprouxVincent CoudercChristelle Lesvigne-BuyAude RoyVincent TombelaineJean-Marc BlondyPhilippe RoyChristine RestoinPierre-Olivier Martin
• Organismes : PHOTONIQUE PHOTONIQUE PHOTONIQUE PHOTONIQUE PHOTONIQUE PHOTONIQUE PHOTONIQUE PHOTONIQUE XLIM

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Xavier ThuruD Philippe
• Organismes : Physiopathologie des Maladies Inflammatoires de l'Intestin Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837 Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers = Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277 Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer - U837
• Publié dans Gut le 25/10/2020

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• Type de publi. : Communication dans un congrès
• Date de publi. : 01/06/2006
• Auteurs : Matthieu AngeliJean-Philippe BigasBeatriz MenendezRonan L. HébertChristian David
• Organismes : Laboratoire de tectonique Laboratoire de mécanique et matériaux du génie civil Laboratoire de tectonique Laboratoire de tectonique Laboratoire de tectonique

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Résumé : The importance of capillary imbibition and evaporation processes in the decay of stone through salt crystallization is estimated by different experiments adapted from the European standard EN 12370. The various tests consist in slightly modifying the salt (sodium sulphate) supply in the porous network of stones as well as the amount of evaporation and in checking the weathering evolution of the stone. The results show that ionic diffusion and dissolution of pre-existent salts are not efficient enough to imply supersaturation, hence to visually alter the stone. An exterior supply of salt is required. The influence of evaporation is clearly shown when evaporation process could not occur through a side of the sample. In this case, isolated sides are never subjected to weathering, even to simple salt efflorescences

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Fichiers liés : Influence_of_capillary_properties_and_evaporation_on_salt_weathering_of_sedimentary_rocks_-_HWC_2006.pdf

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• Type de publi. : Communication dans un congrès
• Date de publi. : 01/06/2006
• Auteurs : Matthieu AngeliJean-Philippe BigasBeatriz MenendezRonan L. HébertChristian David
• Organismes : Laboratoire de tectonique Laboratoire de mécanique et matériaux du génie civil Laboratoire de tectonique Laboratoire de tectonique Laboratoire de tectonique

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Résumé : L'importance de l'imbibition capillaire et des processus d'évaporation dans l'altération des pierres de construction sous l'action de la cristallisation des sels solubles est estimée par différents modes opératoires adaptés de la norme européenne EN 12370. Les différents tests consistent à modifier les conditions de l'apport en sel (sulfate de sodium) dans le réseau poreux des pierres ainsi que les possibilités d'évaporation, et de suivre l'évolution de la détérioration. Les résultats montrent que la dissolution de sels en quantité insuffisante dans le réseau poreux n'altère pas la pierre. Un apport extérieur de sels est nécessaire. L'influence de l'évaporation est aussi clairement démontrée par la localisation des dégâts dans les zones non-isolées

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Fichiers liés : Alteration_par_les_sels_des_pierres_de_construction_-_AUGC_2006.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Anne ClavreulLaurence SindjiAnne Aubert-PouësselJean-Pierre BenoîtPhilippe MeneiC.N. Montero-Menei
• Organismes : Ingénierie de la vectorisation particulaire Service de neurochirurgie [CHU Angers] Ingénierie de la vectorisation particulaire Ingénierie de la vectorisation particulaire Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier Ingénierie de la vectorisation particulaire Laboratoire de thermodynamique et physico-chimie métallurgiques Ingénierie de la vectorisation particulaire Service de neurochirurgie [CHU Angers] Institut Gilbert-Laustriat : Biomolécules, Biotechnologie, Innovation Thérapeutique Ingénierie de la vectorisation particulaire
• Publié dans European Journal of Pharmaceutics and Biopharmaceutics le 22/10/2020

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Résumé : The transplantation of fetal ventral mesencephalic (FVM) cell suspensions into the brain striatal system is an alternative approach for the treatment of Parkinson's disease (PD). However, one objection to this procedure is the relatively poor survival of implanted cells. Attempts have been made to improve the survival of grafted dopaminergic neurons using glial cell line-derived neurotrophic factor (GDNF). Nevertheless, the clinical application of GDNF is limited, due to the difficulties in administering a protein to the brain tissue and due to the ubiquity of its receptor, thus leading to neurological side effects. A strategy to deliver GDNF in the brain based on the intracerebral implantation of biodegradable poly(D,L-lactic acid-co-glycolic acid) sustained release microspheres has been developed. Such microparticles can be easily implanted by sterotaxy in precise and functional areas of the brain without causing damage to the surrounding tissue. Moreover, the release profile of the GDNF-loaded microspheres showed a sustained release over 56 days of biologically active GDNF at clinically relevant doses. The present study shows that the implantation of GDNF-loaded microspheres at a distance to the site of FVM cells in the 6-hydroxydopamine-lesioned rat model of PD improves dopaminergic graft survival and function. Furthermore, the unloaded and the GDNF-loaded microspheres, when they are mixed with FVM cells, may provide a mechanical support and a 3D environment inducing differentiation and increased function of dopaminergic neurons. Taken together, these results show that GDNF microspheres represent an efficient delivery system for cell transplantation studies.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Thorkild I. A. SørensenPhilippe BoutinMoira A TaylorLesli H LarsenCamilla VerdichLiselotte PetersenClaus HolstSøren M EchwaldChristian DinaSøren ToubroMartin PetersenJan PolakKarine ClémentJ Alfredo MartínezDominique LanginJean-Michel OppertVladimir StichIan A. MacdonaldPeter ArnerWim H. M. SarisOluf PedersenArne AstrupPhillippe Froguel
• Organismes : Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Centre de recherche cerveau et cognition Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Laboratoire de Mécanique de Lille - FRE 3723 Génétique des maladies multifactorielles Déterminants Biologiques et Comportementaux des Obésités Service de nutrition Nutrition et obésité : approches génétique et transcriptomique Département de nutrition Laboratoire des Amino-acides Peptides et Protéines Centre d'études spatiales de la biosphère Gestion environnementale et traitement biologique des déchets Systèmes Macromoléculaires et Physiopathologie Humaine Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] Génétique des maladies multifactorielles Unité de recherche sur les obésités Génétique des maladies multifactorielles Déterminants Biologiques et Comportementaux des Obésités Département de nutrition Génétique des maladies multifactorielles Department of Sport Medicine Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Génétique des maladies multifactorielles Section of Genomic Medicine Genome Centre
• Publié dans PLoS clinical trials le 27/10/2020

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Résumé : OBJECTIVES: To study if genes with common single nucleotide polymorphisms (SNPs) associated with obesity-related phenotypes influence weight loss (WL) in obese individuals treated by a hypo-energetic low-fat or high-fat diet. DESIGN: Randomised, parallel, two-arm, open-label multi-centre trial. SETTING: Eight clinical centres in seven European countries. PARTICIPANTS: 771 obese adult individuals. INTERVENTIONS: 10-wk dietary intervention to hypo-energetic (-600 kcal/d) diets with a targeted fat energy of 20%-25% or 40%-45%, completed in 648 participants. OUTCOME MEASURES: WL during the 10 wk in relation to genotypes of 42 SNPs in 26 candidate genes, probably associated with hypothalamic regulation of appetite, efficiency of energy expenditure, regulation of adipocyte differentiation and function, lipid and glucose metabolism, or production of adipocytokines, determined in 642 participants. RESULTS: Compared with the noncarriers of each of the SNPs, and after adjusting for gender, age, baseline weight and centre, heterozygotes showed WL differences that ranged from -0.6 to 0.8 kg, and homozygotes, from -0.7 to 3.1 kg. Genotype-dependent additional WL on low-fat diet ranged from 1.9 to -1.6 kg in heterozygotes, and from 3.8 kg to -2.1 kg in homozygotes relative to the noncarriers. Considering the multiple testing conducted, none of the associations was statistically significant. CONCLUSIONS: Polymorphisms in a panel of obesity-related candidate genes play a minor role, if any, in modulating weight changes induced by a moderate hypo-energetic low-fat or high-fat diet.

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Fichiers liés : pctr.0010012.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Kaveh SaremiJon MarehbianXiaohong YanJean-Philippe RegnauxRobert ElashoffBernard BusselBruce H Dobkin
• Organismes : Institute of VLSI Design Department of Neurology Physiologie et physiopathologie de la motricité chez l'homme Physiologie et physiopathologie de la motricité chez l'homme Department of Neurological Rehabilitation
• Publié dans Journal of Neurologic Rehabilitation le 27/10/2020

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Résumé : OBJECTIVE: Measures of walking ability in large clinical trials are usually limited to a timed short-distance walk and the distance walked in a fixed time. A new integrated system of 5 accelerometers was tested for reliability and compared to a footswitch system to determine if the accelerometers offered a practical option for the acquisition of spatiotemporal gait parameters. METHODS: Leg accelerations and decelerations were defined in relation to simultaneous kinematic and electromyographic data acquired from a healthy subject. Eight healthy adults and 6 independent ambulators with hemiparetic stroke walked 15 m at 2 different speeds wearing both the accelerometers and footswitches. Twelve healthy subjects walked at 5 different speeds repeated 3 times on each of 2 days wearing the accelerometers. Walking speed, cadence, stride length, and single- and double-limb support, swing, and stance times were calculated. RESULTS: No differences (t test, P > 0.2) were found between footswitch and accelerometer variables when comparing all left or right legs in healthy subjects and all paretic or unaffected legs in stroke subjects. A 2-way nested ANOVA model (speed, left and right legs, trial, and session) with the accelerometers at walking speeds from 0.5 to 1.8 m/s revealed high reproducibility of all measures. CONCLUSIONS: The accelerometry system provided reliable and valid spatiotemporal measures of gait for the upper range of speeds likely to be targeted for rehabilitation interventions in ambulatory subjects.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Eric ToussirotÉric RobinetPhilippe SaasJacqueline ChabodBenoit AugéGabriel CozmaPierre TiberghienJean RoudierDaniel Wendling
• Organismes : Agents pathogènes et inflammation - UFC (EA 4266) Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Centre de biologie du développement Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Immunogenetique de la Polyarthrite Rhumatoide Agents pathogènes et inflammation - UFC (EA 4266)
• Publié dans Autoimmunity le 28/10/2020

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Résumé : The Escherichia Coli bacterial extract (OM-89) is used in the treatment of rheumatoid arthritis (RA). We evaluated the immunological changes induced by oral administration of OM-89 in 12 RA patients (polyclonal T cell reactivity to PHA, T cell precursor frequencies specific for OM-89 and Tetanus toxoid (TT), a control antigen and the release of Th1 (IFN-gamma, TNF-alpha), Th2 (IL-4) and T regulatory 1 cell (Tr1) (IL-10) cytokines in the supernatants of PBMC cultures. Stimulation index in response to PHA decreased at month 3 as well as T cell precursor frequencies specific for TT with similar trends for OM-89-specific T cell precursor frequencies. OM-89 induced a strong production of IL-10, a significant decrease in IL-4 production while TNF-alpha and IFN-gamma production tended to decrease during the study. Our results suggest that OM-89 has immunomodulatory properties by inducing changes in PBMC cytokines release suggestive of an induced Tr1 response to OM-89.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/06/2006
• Auteurs : Emilie DubuissonPhilippe LavieFrancis DalardJean-Pierre CaireSabine Szunerits
• Organismes : Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces Laboratoire d'Electrochimie et de Physico-chimie des Matériaux et des Interfaces
• Publié dans Electrochemistry Communications le 02/11/2020

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Résumé : The local corrosion rate of galvanised steel under a thin electrolyte layer comprising chloride and sulphate ions has been investigated. Localised measurements were achieved through the controlled deposition of an electrolytic droplet onto the surface of the sample, into which reference and counter electrodes were inserted to form an electrochemical microcell. The maximal height of the deposited droplet varied between 400 μm and 1100 μm, with a total diameter between 1.34 and 3.35 mm, respectively. Electrochemical polarization curves as well as electrochemical impedance spectroscopy data were recorded on the galvanised steel sample exposed to the electrolytic droplet to determine the local corrosion rate. Corrosion rates were greatly accelerated under electrolyte layers of thicknesses smaller than 800 μm. This increase in corrosion rate is a result of the decrease of the diffusion layer thickness leading to an enhanced rate of oxygen reduction. The corrosion rate of galvanised steel did not alter significantly for electrolytic droplets higher than 800 μm.

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