Philippe JEAN-PIERRE

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2024
• Auteurs : Pierre VauclareJip WulffeléFrançoise LacroixPascale ServantFabrice ConfalonieriJean-Philippe KlemanDominique BourgeoisJoanna Timmins
• Organismes : Institut de biologie structurale Institut de biologie structurale Institut de biologie structurale Institut de Biologie Intégrative de la Cellule Institut de Biologie Intégrative de la Cellule Institut de biologie structurale Institut de biologie structurale Institut de biologie structurale
• Publié dans Nucleic Acids Research le 26/10/2020

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Résumé : Bacteria have developed a wide range of strategies to respond to stress, one of which is the rapid large-scale reorganization of their nucleoid. Nucleoid associated proteins (NAPs) are believed to be major actors in nucleoid remodeling, but the details of this process remain poorly understood. Here, using the radiation resistant bacterium D. radiodurans as a model, and advanced fluorescence microscopy, we examined the changes in nucleoid morphology and volume induced by either entry into stationary phase or exposure to UV-C light, and characterized the associated changes in mobility of the major NAP in D. radiodurans, the heat-unstable (HU) protein. While both types of stress induced nucleoid compaction, HU diffusion was reduced in stationary phase cells, but was instead increased following exposure to UV-C, suggesting distinct underlying mechanisms. Furthermore, we show that UV-C-induced nucleoid remodeling involves a rapid nucleoid condensation step associated with increased HU diffusion, followed by a slower decompaction phase to restore normal nucleoid morphology and HU dynamics, before cell division can resume. These findings shed light on the diversity of nucleoid remodeling processes in bacteria and underline the key role of HU in regulating this process through changes in its mode of assembly on DNA.

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Fichiers liés : Vauclare et al. NAR.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2024
• Auteurs : Yuuki WadaPhilippe LaurentDamien PailotIon CojocariÉric BréelleStéphane ColongesJean-Pierre BaronickFrançois LebrunPierre-Louis BlellyDavid SarriaKazuhiro NakazawaMiles Lindsey Clark
• Organismes : Commissariat à l'énergie atomique et aux énergies alternatives AstroParticule et Cosmologie AstroParticule et Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers AstroParticule et Cosmologie AstroParticule et Cosmologie AstroParticule et Cosmologie Commissariat à l'énergie atomique et aux énergies alternatives AstroParticule et Cosmologie Institut de recherche en astrophysique et planétologie AstroParticule et Cosmologie
• Publié dans Journal of Astronomical Telescopes Instruments and Systems le 01/11/2020

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Résumé : We developed the X-ray, Gamma-ray and Relativistic Electron detector (XGRE) onboard the TARANIS satellite, to investigate high-energy phenomena associated with lightning discharges such as terrestrial gamma-ray flashes and terrestrial electron beams. XGRE consisted of three sensors. Each sensor has one layer of LaBr$_{3}$ crystals for X-ray/gamma-ray detections, and two layers of plastic scintillators for electron and charged-particle discrimination. Since 2018, the flight model of XGRE was developed, and validation and calibration tests, such as a thermal cycle test and a calibration test with the sensors onboard the satellite were performed before the launch of TARANIS on 17 November 2020. The energy range of the LaBr$_{3}$ crystals sensitive to X-rays and gamma rays was determined to be 0.04-11.6 MeV, 0.08-11.0 MeV, and 0.08-11.3 MeV for XGRE1, 2, and 3, respectively. The energy resolution at 0.662 MeV (full width at half maximum) was to be 20.5%, 25.9%, and 28.6%, respectively. Results from the calibration test were then used to validate a simulation model of XGRE and TARANIS. By performing Monte Carlo simulations with the verified model, we calculated effective areas of XGRE to X-rays, gamma rays, electrons, and detector responses to incident photons and electrons coming from various elevation and azimuth angles.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/01/2024
• Auteurs : G. 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• Organismes : Centre de Physique des Particules de Marseille Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire d'Annecy de Physique des Particules Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Centre de Physique des Particules de Marseille Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire d'Annecy de Physique des Particules AstroParticule et Cosmologie Centre de Physique des Particules de Marseille AstroParticule et Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique de Clermont Laboratoire de Physique de Clermont Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique de Clermont Laboratoire de Physique de Clermont Centre de Physique des Particules de Marseille Laboratoire de Physique Nucléaire et de Hautes Énergies Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Centre de Physique des Particules de Marseille Centre de Physique des Particules de Marseille Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique de Clermont Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Subatomique et de Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique de Clermont Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire d'Annecy de Physique des Particules Centre de Physique des Particules de Marseille Centre de Physique des Particules de Marseille Laboratoire de Physique de Clermont Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Centre de Physique des Particules de Marseille Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique Nucléaire et de Hautes Énergies Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire d'Annecy de Physique des Particules Centre de Physique des Particules de Marseille Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Subatomique et de Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique Subatomique et de Cosmologie Laboratoire d'Annecy de Physique des Particules Laboratoire d'Annecy de Physique des Particules AstroParticule et Cosmologie AstroParticule et Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique de Clermont Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Subatomique et de Cosmologie Centre de Physique des Particules de Marseille Laboratoire des deux Infinis de Toulouse Institut de Recherches sur les lois Fondamentales de l'Univers AstroParticule et Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Centre de Physique des Particules de Marseille Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Centre de Physique des Particules de Marseille AstroParticule et Cosmologie Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique de Clermont Centre de Physique des Particules de Marseille Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Nucléaire et de Hautes Énergies Centre de Physique des Particules de Marseille Laboratoire des deux Infinis de Toulouse Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique de Clermont Laboratoire d'Annecy de Physique des Particules Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique Subatomique et de Cosmologie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie AstroParticule et Cosmologie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique de Clermont Centre de Physique des Particules de Marseille Laboratoire des deux Infinis de Toulouse Centre de Physique des Particules de Marseille Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Centre de Physique des Particules de Marseille Centre de Physique des Particules de Marseille Laboratoire de Physique de Clermont Centre de Physique des Particules de Marseille Laboratoire des deux Infinis de Toulouse Laboratoire de Physique Nucléaire et de Hautes Énergies Laboratoire de Physique Subatomique et de Cosmologie Centre de Physique des Particules de Marseille Laboratoire des deux Infinis de Toulouse Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie Laboratoire de Physique de Clermont Institut de Recherches sur les lois Fondamentales de l'Univers Institut de Recherches sur les lois Fondamentales de l'Univers Laboratoire de Physique des 2 Infinis Irène Joliot-Curie AstroParticule et Cosmologie Laboratoire de Physique des 2 Infinis Irène Joliot-Curie
• Publié dans JINST le 08/11/2025

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Résumé : This paper presents the electron and photon energy calibration obtained with the ATLAS detector using 140 fb$^{-1}$ of LHC proton-proton collision data recorded at $\sqrt{s}=13$ TeV between 2015 and 2018. Methods for the measurement of electron and photon energies are outlined, along with the current knowledge of the passive material in front of the ATLAS electromagnetic calorimeter. The energy calibration steps are discussed in detail, with emphasis on the improvements introduced in this paper. The absolute energy scale is set using a large sample of $Z$-boson decays into electron-positron pairs, and its residual dependence on the electron energy is used for the first time to further constrain systematic uncertainties. The achieved calibration uncertainties are typically 0.05% for electrons from resonant $Z$-boson decays, 0.4% at $E_\text{T}\sim 10$ GeV, and 0.3% at $E_\text{T}\sim 1$ TeV; for photons at $E_\text{T}\sim 60$ GeV, they are 0.2% on average. This is more than twice as precise as the previous calibration. The new energy calibration is validated using $J/\psi \to ee$ and radiative $Z$-boson decays.

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