Philippe JEAN-PIERRE

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• Type de publi. : Communication dans un congrès
• Date de publi. : 04/09/2023
• Auteurs : Philippe Andrey
• Organismes : Institut Jean-Pierre Bourgin - Sciences du végétal

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• Type de publi. : Article dans une revue
• Date de publi. : 04/09/2023
• Auteurs : Pierre NivoitThomas MathivetJunxi WuYann SalemkourDevanarayanan Siva SankarVéronique BaudrieJennifer BourreauAnne-Laure GuihotEmilie VessieresMathilde LemitreCinzia BoccaJérémie TeillonMorgane Le GallAnna ChipontEstelle RobidelNeeraj DhaunEric CamererPascal ReynierEtienne RouxThierry CouffinhalPatrick W F HadokeJean-Sébastien SilvestreXavier GuillonneauPhilippe BonninDaniel HenrionJoern DengjelPierre-Louis TharauxOlivia Lenoir
• Organismes : Bordeaux Imaging Center Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases Biologie des maladies cardiovasculaires = Biology of Cardiovascular Diseases
• Publié dans Cellular and Molecular Life Sciences le 29/10/2020

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Résumé : Correction: Cellular and Molecular Life Sciences (2023) 80:210 https://doi.org/10.1007/s00018-023-04859-9

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Fichiers liés : BMC_CellMolLifeSci_2023_Nivoit.pdf

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• Type de publi. :
• Date de publi. : 04/09/2023
• Auteurs : Alexandre DurrmeyerJean-Christophe PalauquiPhilippe Andrey
• Organismes : Institut Jean-Pierre Bourgin - Sciences du végétal Université Paris-Saclay AgroParisTech Institut Jean-Pierre Bourgin - Sciences du végétal Université Paris-Saclay AgroParisTech Institut Jean-Pierre Bourgin - Sciences du végétal Université Paris-Saclay AgroParisTech

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Résumé : Plant embryo development is an intricate process whereby a complex organism arises from a single cell. At the cellular scale, this process hinges on the fundamental mechanisms of cell division and growth. Cell division, in particular, plays a pivotal role by reshaping tissue properties like mechanical constraints and topology, thereby influencing morphology. Predicting the positioning of a new cell wall during division is thus crucial for understanding morphogenesis. Early attempts to predict cell division positioning relied on geometric approaches based on the shape of the mother cell. While geometry contributes to division plane positioning, other factors like chemical signaling, mechanical constraints, and tissue-scale optimizations also exert influence. However, deciphering how each factor impacts division remains challenging. Current models, whether based on geometry, stresses, or other properties, are typically rule-based, incorporating manually defined rules that may contain human biases or overlook certain aspects. It is then difficult to define whether the data does not fit any rule or if the right rule was simply not identified. In A. thaliana embryos, a geometric division rule successfully fit the observed divisions from stage 1C to stage 32C, with the exception of the stage 16C basal intern cells (Moukhtar et al. 2019, Laruelle et al. 2022). Do these latter cells follow a different rule from the previous stages, or is there an alternative common rule? To ensure that the proposed division rule aligns as closely as possible with observations, we propose a method to automatically learn predicting cell division plane positioning in a supervised framework. Leveraging U-Net3D, a deep learning architecture tailored for biological image segmentation, we train a model to approximate division rules based on an image dataset of before/after division pairs. This formulation transforms the prediction of division plane positioning into a more accessible segmentation task. Additionally, representing mother cells by their 3D image masks enables the model to approximate a strict geometric division rule from fully preserved geometric information. We conducted multiple experiments to assess the potential and limitations of this novel approach. We first evaluated the network's ability to learn a simple division rule using synthetic shapes like cuboids and ellipsoids. Subsequently, we tested the model's generalization capability across different shapes and its performance in approximating complex division rules. Finally, we applied the approach to real A. thaliana embryo cells spanning developmental stages 1C to 32C. We trained the model to learn stage-specific division rules and subsequently a universal rule applicable across all stages.

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Fichiers liés : Poster.pdf

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• Type de publi. :
• Date de publi. : 03/09/2023
• Auteurs : Annia Alba MenéndezR. ScholsAntonio VazquezJ. SánchezPhilippe DouchetM. SanchezDominique FaugereJean-Pierre PointierSylvie Hurtrez-BoussèsT. HuyseOlivier ReyBenjamin Gourbal
• Organismes : Instituto de Medicina Tropical “Pedro Kourí” / Pedro Kouri Institute of Tropical Medicine [Havana, Cuba] Interactions Hôtes-Pathogènes-Environnements Interactions Hôtes-Pathogènes-Environnements Interactions Hôtes-Pathogènes-Environnements CISDEM-CSIC Centre de recherches insulaires et observatoire de l'environnement Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle Interactions Hôtes-Pathogènes-Environnements Interactions Hôtes-Pathogènes-Environnements

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• Type de publi. : Article dans une revue
• Date de publi. : 02/09/2023
• Auteurs : Julien VaidieEdwige PejuLouise-Marie JandeauxMathieu LesouhaitierJean-Claude LacheradeAntoine GuillonXavier WittebolePierre AsfarBruno EvrardThomas DaixPhilippe VignonBruno François
• Organismes : Service de Réanimation Polyvalente [CHU Limoges] Hôpital Cochin [AP-HP] Nouvel Hôpital Civil de Strasbourg Centre Hospitalier Universitaire de Rennes [CHU Rennes] = Rennes University Hospital [Pontchaillou] Centre Hospitalier Départemental - Hôpital de La Roche-sur-Yon Centre d’Etude des Pathologies Respiratoires [Tours] Université de Tours Centre Hospitalier Régional Universitaire de Tours Cliniques universitaires St Luc [Bruxelles] Centre Hospitalier Universitaire d'Angers Centre d'Investigation Clinique de Limoges Centre Hospitalier Universitaire Dupuytren 1 et 2 Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques Centre d'Investigation Clinique de Limoges Centre Hospitalier Universitaire Dupuytren 1 et 2 Centre d'Investigation Clinique de Limoges Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques Centre Hospitalier Universitaire Dupuytren 1 et 2 Centre d'Investigation Clinique de Limoges Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques Centre Hospitalier Universitaire Dupuytren 1 et 2
• Publié dans Critical Care le 31/10/2020

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Résumé : Abstract Background Except in a few retrospective studies mainly including patients under chemotherapy, information regarding the impact of immunosuppressive therapy on the prognosis of patients admitted to the intensive care unit (ICU) for septic shock is scarce. Accordingly, the PACIFIC study aimed to asses if immunosuppressive therapy is associated with an increased mortality in patients admitted to the ICU for septic shock. Methods This was a retrospective epidemiological multicentre study. Eight high enroller centres in septic shock randomised controlled trials (RCTs) participated in the study. Patients in the “exposed” group were selected from the screen failure logs of seven recent RCTs and excluded because of immunosuppressive treatment. The “non-exposed” patients were those included in the placebo arm of the same RCTs. A multivariate logistic regression model was used to estimate the risk of death. Results Among the 433 patients enrolled, 103 were included in the “exposed” group and 330 in the “non-exposed” group. Reason for immunosuppressive therapy included organ transplantation (n = 45 [44%]) or systemic disease (n = 58 [56%]). ICU mortality rate was 24% in the “exposed” group and 25% in the “non-exposed” group ( p = 0.9). Neither in univariate nor in multivariate analysis immunosuppressive therapy was associated with a higher ICU mortality (OR: 0.95; [95% CI 0.56–1.58]: p = 0.86 and 1.13 [95% CI 0.61–2.05]: p = 0.69, respectively) or 3-month mortality (OR: 1.13; [95% CI 0.69–1.82]: p = 0.62 and OR: 1.36 [95% CI 0.78–2.37]: p = 0.28, respectively). Conclusions In this study, long-term immunosuppressive therapy excluding chemotherapy was not associated with significantly higher or lower ICU and 3-month mortality in patients admitted to the ICU for septic shock.

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Fichiers liés : s13054-023-04626-z.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/09/2023
• Auteurs : Alexia BodinLogan GreibillJulien GoujuFranck LetournelSilvia PozziJean-Pierre JulienLaurence RenaudDelphine BohlStéphanie MillecampsChristophe VernyJulien CassereauGuy LenaersArnaud ChevrollierAnne-Marie TassinPhilippe Codron
• Organismes : MitoVasc - Équipe MitoLab (Mitochondrial Pathophysiology) Institut de Biologie Intégrative de la Cellule Centre Hospitalier Universitaire d'Angers Centre Hospitalier Universitaire d'Angers Département de Psychiatrie et Neurosciences [Université Laval] = Department of Psychiatry and Neuroscience [University of Laval] Centre de recherche de l’Institut universitaire en santé mentale de Québec [Canada] Département de Psychiatrie et Neurosciences [Université Laval] = Department of Psychiatry and Neuroscience [University of Laval] Centre de recherche de l’Institut universitaire en santé mentale de Québec [Canada] Département de Psychiatrie et Neurosciences [Université Laval] = Department of Psychiatry and Neuroscience [University of Laval] Groupe de recherche sur le système nerveux central [Montréal] Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute Centre Hospitalier Universitaire d'Angers MitoVasc - Équipe MitoLab (Mitochondrial Pathophysiology) Centre Hospitalier Universitaire d'Angers MitoVasc - Équipe MitoLab (Mitochondrial Pathophysiology) MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale Centre Hospitalier Universitaire d'Angers MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale Institut de Biologie Intégrative de la Cellule MitoVasc - Physiopathologie Cardiovasculaire et Mitochondriale Centre Hospitalier Universitaire d'Angers Centre Hospitalier Universitaire d'Angers
• Publié dans Brain - A Journal of Neurology le 29/10/2020

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Résumé : Abstract The centrosome, as the main microtubule organizing centre, plays key roles in cell polarity, genome stability and ciliogenesis. The recent identification of ribosomes, RNA-binding proteins and transcripts at the centrosome suggests local protein synthesis. In this context, we hypothesized that TDP-43, a highly conserved RNA binding protein involved in the pathophysiology of amyotrophic lateral sclerosis and frontotemporal lobar degeneration, could be enriched at this organelle. Using dedicated high magnification sub-diffraction microscopy on human cells, we discovered a novel localization of TDP-43 at the centrosome during all phases of the cell cycle. These results were confirmed on purified centrosomes by western blot and immunofluorescence microscopy. In addition, the co-localization of TDP-43 and pericentrin suggested a pericentriolar enrichment of the protein, leading us to hypothesize that TDP-43 might interact with local mRNAs and proteins. Supporting this hypothesis, we found four conserved centrosomal mRNAs and 16 centrosomal proteins identified as direct TDP-43 interactors. More strikingly, all the 16 proteins are implicated in the pathophysiology of TDP-43 proteinopathies, suggesting that TDP-43 dysfunction in this organelle contributes to neurodegeneration. This first description of TDP-43 centrosomal enrichment paves the way for a more comprehensive understanding of TDP-43 physiology and pathology.

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• Type de publi. : Chapître d'ouvrage
• Date de publi. : 01/09/2023
• Auteurs : Muriel GandelinPhilippe GouézinIvy Thomson
• Organismes : Institut national de recherches archéologiques préventives Travaux et recherches archéologiques sur les cultures, les espaces et les sociétés Centre de Recherche en Archéologie, Archéosciences, Histoire Institut national de recherches archéologiques préventives

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• Type de publi. :
• Date de publi. : 01/09/2023
• Auteurs : Bru PPhilippe PointereauChavre ATondusson JIsabelle LegrandJean-François HocquetteMarie-Pierre Ellies-Oury
• Organismes : Unité Mixte de Recherche sur les Herbivores - UMR 1213 Unité Mixte de Recherche sur les Herbivores - UMR 1213 Ecole Nationale Supérieure des Sciences Agronomiques de Bordeaux-Aquitaine

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• Type de publi. : Article dans une revue
• Date de publi. : 01/09/2023
• Auteurs : Pauline Lallemant-DudekLivia ParodiGiulia CoarelliAnna HeinzmannPerrine CharlesClaire EwenczykSilvia FenuMarie-Lorraine MoninPhilippe CorciaChristel DepienneFanny MochelJean BenardSophie Tezenas Du MontcelAlexandra Dürr
• Organismes : Sorbonne Université Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute CHU Pitié-Salpêtrière [AP-HP] CHU Pitié-Salpêtrière [AP-HP] Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute Centre Hospitalier Régional Universitaire de Tours Institut du Cerveau = Paris Brain Institute Institut du Cerveau = Paris Brain Institute Association Strümpell-Lorrain Algorithms, models and methods for images and signals of the human brain = Algorithmes, modèles et méthodes pour les images et les signaux du cerveau humain [ICM Paris] Institut Pierre Louis d'Epidémiologie et de Santé Publique
• Publié dans Annals of Physical and Rehabilitation Medicine le 02/11/2020

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Résumé : Background: Phenotypic variability is a consistent finding in neurogenetics and therefore applicable to hereditary spastic paraparesis. Identifying reasons for this variability is a challenge. We hypothesized that, in addition to genetic modifiers, extrinsic factors influence variability. Objectives: Our aim was to describe the clinical variability in hereditary spastic paraparesis from the person's perspective. Our goals were to identify individual and environmental factors that influence muscle tone disorders and derive interventions which could improve spasticity. Methods: This study was based on self-assessments with questions on nominal and ordinal scales completed by participants with hereditary spastic paraparesis. A questionnaire was completed either in-person in the clinic or electronically via lay organization websites. Results: Among the 325 responders, most had SPG4/SPAST (n = 182, 56%) with a mean age at onset of 31.7 (SD 16.7) years and a mean disease duration of 23 (SD 13.6) years at the time of participation. The 2 factors identified as improving spasticity for > 50% of the responders were physiotherapy (193/325, 59%), and superficial warming (172/308, 55%). Half of the responders (n = 164, 50%) performed physical activity at least once a month and up to once a week. Participants who reported physiotherapy as effective were significantly more satisfied with ≥ 3 sessions per week. Psychologically stressful situations (246/319, 77%) and cold temperatures (202/319, 63%) exacerbated spasticity for most participants. Conclusion: Participants perceived that physiotherapy reduced spasticity and that the impact of physiotherapy on spasticity was much greater than other medical interventions. Therefore, people should be encouraged to practice physical activity at least 3 times per week. This study reported participants' opinions: in hereditary spastic paraparesis only functional treatments exist, therefore the participant's expertise is of particular importance.

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Fichiers liés : AnnalesMPR_Modifspa_manuscript.v2.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 01/09/2023
• Auteurs : Justyna Elżbieta JońcaKevin Castelló LuxKatia FajerwergMyrtil L KahnV. CollièrePhilippe MeniniIzabela SówkaPierre Fau
• Organismes : Wroclaw University of Science and Technology = Politechnika Wroclawska [Wrocław] Laboratoire de chimie de coordination Laboratoire de chimie de coordination Laboratoire de chimie de coordination Laboratoire de chimie de coordination Équipe MICrosystèmes d'Analyse Wroclaw University of Science and Technology = Politechnika Wroclawska [Wrocław] Laboratoire de physique et chimie des nano-objets
• Publié dans Chemosensors le 21/10/2020

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Résumé : The nanometer size Cu2O@WO3·H2O composite material has been prepared by the direct hydrolysis of mesitylcopper (I) on WO3·2H2O nanoleaves. The synthesis has been performed in toluene without the addition of any ancillary ligands. The prepared nanocomposite has been deposited as a gas-sensitive layer on miniaturized silicon devices and heated up gradually to 500 °C in the ambient air. During the heating, the CuWO4 phase is formed upon the reaction of Cu2O with the WO3 support as revealed by the XRD analyses. The as-prepared CuWO4@WO3 sensors have been exposed to 10 ppm of CO or 0.4 ppm of NO2 (RH = 50%). At the operating temperature of 445 °C, a normalized response of 620% towards NO2 is obtained whereas the response to CO is significantly lower (S = 30%). Under these conditions, the sensors prepared either with pristine CuO or WO3 nanostructures are sensitive to only one of the two investigated gases, i.e., CO and NO2, respectively. Interestingly, when the CuWO4@WO3 sensitive layer is exposed to UV light emitted from a 365 nm Schottky diode, its sensitivity towards CO vanishes whereas the response towards NO2 remains high. Thus, the application of UV illumination allowed us to modify the selectivity of the device. This new nanocomposite sensor is a versatile sensitive layer that will be integrated into a gas sensor array dedicated to electronic nose platforms.

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Fichiers liés : Jońca, Gas sensing properties, 2023.pdf

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