Philippe JEAN-PIERRE

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• Type de publi. : Article dans une revue
• Date de publi. : 01/05/2006
• Auteurs : Emmanuel MessasAlain BelMiguel Cortes MorichettiClaire CarrionMarc HandschumacherSéverine PeyrardJean-Thomas VilquinMichel DesnosPatrice BrunevalAlain CarpentierPhilippe MenaschéRobert LevineAlbert Hagège
• Organismes : Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Physiopathologie et thérapie du muscle strié Institut de Myologie CHU Pitié-Salpêtrière [AP-HP] Harvard Medical School [Boston] CIC - HEGP Physiopathologie et thérapie du muscle strié Institut de Myologie CHU Pitié-Salpêtrière [AP-HP] Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Hôpital Européen Georges Pompidou [APHP] Immunopathologie humaine Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP] Harvard Medical School [Boston] Therapie Cellulaire en Pathologie Cardio-Vasculaire Hôpital Européen Georges Pompidou [APHP]
• Publié dans Journal of the American College of Cardiology le 26/10/2020

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Résumé : Objectives: This study was designed to assess whether post-myocardial infarction (MI) in-scar transplantation of skeletal myoblasts (SM) could reduce chronic ischemic mitral regurgitation (MR) by decreasing left ventricular (LV) remodeling. Background: Extensive work has confirmed the relationship between ischemic MR and post-myocardial infarction (MI) remodeling of the LV. Methods: An infero-posterior MI was created in 13 sheep, thereby resulting in increasing MR. Two months post-MI, the animals were randomized and in-scar injected with expanded autologous SM (n = 6, mean: 251 x 10(6) cells) or culture medium only (n = 7). Three-dimensional echocardiography was performed at baseline, before transplantation, and for two months thereafter (sacrifice), with measurements of LV end-diastolic and end-systolic volumes (ESV), ejection fraction (EF), MR stroke volume, and leaflet tethering distance; wall motion score index (WMSi) was assessed by two-dimensional echo. Results: Measurements were similar between groups at baseline and before transplantation. At sacrifice, transplantation was found to have reduced MR progression (regurgitant volume change: -1.83 +/- 0.32 ml vs. 5.9 +/- 0.7 ml in control group, p < 0.0001) and tethering distance (-0.41 +/- 0.09 cm vs. 0.44 +/- 0.12 cm in control group, p < 0.001), with significant improvement of EF (2.01 +/- 0.94% vs. -4.86 +/- 2.23%, p = 0.02), WMSi (-0.25 +/- 0.11 vs. 0.13 +/- 0.03 in controls, p < 0.01) and a trend to a lesser increase in ESV (23.3 +/- 3.5 ml vs. 35.4 +/- 4.2 ml in control group, p = 0.055). Conclusions: Autologous skeletal myoblast transplantation attenuates mild-to-moderate chronic ischemic MR, which otherwise is progressive, by decreasing tethering distance and improving EF and wall motion score, thereby enhancing valve coaptation. These data shed additional light on the mechanism by which skeletal myoblast transplantation may be cardioprotective.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/05/2006
• Auteurs : Agnès GoubleJulianne SmithSylvia BruneauChristophe PerezValérie GuyotJean‐pierre CabaniolsSophie LeducLaurence FiettePatrick AvéBéatrice MicheauPhilippe DuchateauFrédéric Pâques
• Organismes : Cellectis Cellectis Cellectis Cellectis Cellectis Cellectis Cellectis Histotechnologie et Pathologie Histotechnologie et Pathologie Cellectis Cellectis Cellectis
• Publié dans The Journal of Gene Medicine le 25/10/2020

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Résumé : Background Sequence‐specific endonucleases with large recognition sites can cleave DNA in living cells, and, as a consequence, stimulate homologous recombination (HR) up to 10 000‐fold. The recent development of artificial meganucleases with chosen specificities has provided the potential to target any chromosomal locus. Thus, they may represent a universal genome engineering tool and seem to be very promising for acute gene therapy. However, in toto applications depend on the ability to target somatic tissues as well as the proficiency of somatic cells to perform double‐strand break (DSB)‐induced HR. Methods In order to investigate DSB‐induced HR in toto , we have designed transgenic mouse lines carrying a LagoZ gene interrupted by one I‐ Sce I cleavage site surrounded by two direct repeats. The LagoZ gene can be rescued upon cleavage by I‐ Sce I and HR between the two repeats in a process called single‐strand annealing. β‐Galactosidase activity is monitored in liver after tail vein injection of adenovirus expressing the meganuclease I‐SceI. Results In toto staining revealed a strong dotted pattern in all animals injected with adenovirus expressing I‐SceI. In contrast, no staining could be detected in the control. β‐Galactosidase activity in liver extract, tissue section staining, and PCR analysis confirmed the presence of the recombined LagoZ gene. Conclusions We demonstrate for the first time that meganucleases can be successfully delivered in animal and induce targeted genomic recombination in mice liver in toto . These results are an essential step towards the use of designed meganucleases and show the high potential of this technology in the field of gene therapy. Copyright © 2006 John Wiley & Sons, Ltd.

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• Type de publi. : Communication dans un congrès
• Date de publi. : 01/05/2006
• Auteurs : C. Camy-PeyretSébastien PayenPascal PrunetEmmanuel DufourRoger BruggeAlan O'NeillJean GirouxPhilippe Lagueux
• Organismes : Laboratoire de Physique Moleculaire pour l'Atmosphere et l'Astrophysique Laboratoire de Physique Moleculaire pour l'Atmosphere et l'Astrophysique NOVELTIS [Sté] NOVELTIS [Sté] University of Reading University of Reading

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Résumé : Because of their high spectral resolution (0.035 cm-1 unapodised), MIPAS spectra contain potential information on the Doppler shifts due to wind along the line-of-sight (LOS) in a given limb scan sequence. The intrinsic absolute stability of the MIPAS wavenumber scale is not granted to the required precision, however. The present ESA operational spectral calibration algorithm is relying on the position of the atmospheric emission lines themselves (assuming no wind effect and in a small range of tangent altitudes where lines are neither too broadened by pressure effects, nor too weak). An algorithm has been proposed that just relies on the relative stability of the wavenumber scale within a given limb scan sequence (usually 17 spectra recorded from about 65 to 10 km in approximately 75 s) to derive a vertical LOS relative wind profile. This LOS wind information is derived by an appropriate algorithm called ANA (for analytical method because an explicit formula is used) producing a wavenumber scaling factor between consecutive spectra in a given limb scan. This scaling factor is derived from a number of optimised wind spectra windows spanning the 5 bands covered by MIPAS. Because of the relative nature of the process, the absolute interband/interwindow calibration is not needed and an optimum weighted average value of the scaling factors can be derived with weights (and an associated occupation matrix) representing the impact of radiometric noise in each window w and for each pair of tangent heights. The average scaling factor is then converted to a relative LOS wind difference and, assuming the LOS wind is known at one level, an absolute LOS wind profile can be provided. The algorithm has been developed (through an ESA contract study), coded and tested on simulated spectra (to check the performances of the method and to establish the noise amplification error factors). We are in the process of testing the algorithm on a set of selected MIPAS spectra recorded in correlation with standard meteorological radio- soundings (providing T, u and v as a function of P). Preliminary results of this study will be presented including comparisons with ECMWF and sonde results, showing that a precision reaching the target of 5 m/s could be expected in the altitude range 20 to 35 km.

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• Type de publi. : Article dans une revue
• Date de publi. : 27/04/2006
• Auteurs : Cécile CourivaudChristophe FerrandMarina DeschampsPierre TiberghienJean-Marc ChalopinAnne DuperrierPhilippe SaasDidier Ducloux
• Organismes : Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Service d'urologie, andrologie et transplantation rénale Plateforme de Biomonitoring Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Plateforme de Biomonitoring Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Plateforme de Biomonitoring Centre d'Investigation Clinique de Besançon Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Service d'urologie, andrologie et transplantation rénale Centre d'Investigation Clinique de Besançon Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Plateforme de Biomonitoring Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Plateforme de Biomonitoring Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) Service d'urologie, andrologie et transplantation rénale Centre d'Investigation Clinique de Besançon
• Publié dans Transplantation le 25/10/2020

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Résumé : Stable renal transplant recipients (RTR) display high rates of atherosclerotic events (AE). Innate immunity and especially vascular inflammation play a role in the pathogenesis of atherosclerosis. It is illustrated both by an increased occurrence of postrenal transplant cardiovascular events in patients with elevated levels of C-reactive protein and by a correlation between posttransplant AE and Toll-like receptor-4 Asp299Gly polymorphism. Here, we analyze the influence NOD2/CARD15 gene polymorphism since NOD2 can modulate macrophage pro-inflammatory activity and macrophage is present in early atherosclerotic lesions. The incidence of single nucleotide polymorphism (SNP) in the three major polymorphic region of NOD2 gene (SNP8, SNP12 and SNP13) was assessed in 182 RTR and the correlation between such polymorphism and the development of AE was analyzed. No correlation was observed between NOD2 gene polymorphism and the occurrence of AE after renal transplantation. NOD2 gene polymorphism thus does not appear to influence cardiovascular complications in RTR.

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Fichiers liés : Courivaud_C_Transplantation_2006.pdf inserm-00473148_edited.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 21/04/2006
• Auteurs : Emilie CollartAbhay ShuklaJean-Pascal RueffPhilippe LeiningerHirofumi IshiiIgnace JarrigeYong CaiSang-Wook CheongGuy Dhalenne
• Organismes : Institut de minéralogie et de physique des milieux condensés Institut de minéralogie et de physique des milieux condensés Laboratoire de Chimie Physique - Matière et Rayonnement Laboratoire de Chimie Physique - Matière et Rayonnement National Synchrotron Radiation Research Center National Synchrotron Radiation Research Center National Synchrotron Radiation Research Center Rutgers, The State University of New Jersey [New Brunswick] Laboratoire de Physico-Chimie de l'État Solide
• Publié dans Physical Review Letters le 02/11/2020

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Résumé : The dynamics of doped charge in an antiferromagnetic lattice is central to the description of the insulator-metal transition that occurs on doping the parent high Tc compounds. In this work we use high resolution resonant inelastic x-ray scattering to investigate the dynamics of the charge-transfer exciton by measuring its energy dispersion in two prototype compounds, La2CuO4 and La2NiO4. We show that this behavior is radically different in the cuprate with respect to a system known to exhibit strong polaronic behavior, namely, the nickelate: the exciton is mobile in the cuprate while it is well localized in the nickelate. Using a simple Wannier-Mott model we can estimate the total hole plus electron effective mass in the cuprate to be 3.5±0.3me which would exclude strong localization in the undoped cuprate.

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Fichiers liés : PhysRevLett.96.157004.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 15/04/2006
• Auteurs : L BollingerPierre HenryJean‐philippe Avouac
• Organismes : Centre Européen de Recherche et d'Enseignement des Géosciences de l'Environnement
• Publié dans Earth and Planetary Science Letters le 01/11/2020

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Résumé : We model crustal deformation and the resulting thermal structure across the Nepal Himalaya, assuming that, since 20 Ma, shortening across the range has been primarily taken up by slip along a single thrust fault, the Main Himalayan Thrust (MHT) Fault, and that the growth of the Himalayan wedge has resulted mainly from underplating and to the development of a duplex at midcrustal depth. We show that this process can account for the inverse thermal metamorphic gradient documented throughout the Lesser Himalaya (LH), the discontinuity of peak metamorphic temperatures across the MCT, as well as the distribution of age of exhumation across the range. This study suggests that the metamorphic evolution of the range over about the last 20 million years is compatible with the kinematics of recent crustal deformation deduced from morphotectonic and geodetic studies.

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• Type de publi. : Article dans une revue
• Date de publi. : 10/04/2006
• Auteurs : Jean-Philippe KarrLaurent Hilico
• Organismes : Laboratoire Kastler Brossel Département de Physique et Modélisation (Evry) Laboratoire Kastler Brossel Département de Physique et Modélisation (Evry)
• Publié dans Journal of Physics B: Atomic, Molecular and Optical Physics le 26/10/2020

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Résumé : We present a nonrelativistic calculation of the rotation-vibration levels of the molecular ions H2+, D2+ and HD+, relying on the diagonalization of the exact three-body Hamiltonian. The J=2 levels are obtained with a very high accuracy of 10^{-14} a.u. (for most levels) representing an improvement by five orders of magnitude over previous calculations. The accuracy is also improved for the J=1 levels of H2+ and D2+ with respect to earlier works. Moreover, we have computed the sensitivities of the energy levels with respect to the mass ratios, allowing these levels to be used for metrological purposes.

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Fichiers liés : Dstates2.pdf

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• Type de publi. : Article dans une revue
• Date de publi. : 06/04/2006
• Auteurs : Leon GengembreBenjamin JarryFranck LaunayJean-Philippe NogierValerie MontouilloutJean-Luc Bonardet
• Organismes : Unité de Catalyse et Chimie du Solide - UMR 8181 Systèmes interfaciaux à l'echelle nanometrique Systèmes interfaciaux à l'echelle nanometrique Systèmes interfaciaux à l'echelle nanometrique Centre de recherches sur les matériaux à haute température Systèmes interfaciaux à l'echelle nanometrique
• Publié dans Applied Catalysis A : General le 27/10/2020

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Résumé : Incorporation or grafting of gallium atoms in/on mesoporous SBA-15 silicas was carried out using four different procedures. The structure and texture of the materials obtained were characterised by classical methods (X-ray diffraction, TEM, porosimetry measurements, XPS, 71Ga NMR spectroscopy). Acidity of the solids was evaluated by ammonia adsorption, TPD of this gas and FTIR spectroscopy of adsorbed probes (2,6-dimethylpyridine and deuterated acetonitrile). Catalytic activity was tested first in the isomerisation of a-pinene and second in the oxidative cleavage of cyclohexene oxide. In this latter case, the results are compared with those obtained in the presence of Al-SBA-15 catalysts prepd. under similar conditions.

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• Type de publi. : Communication dans un congrès
• Date de publi. : 03/04/2006
• Auteurs : Jean-Pierre BarthélemyAngélique LegrainPhilippe LencaBenoît Vaillant
• Organismes : Département Logique des Usages, Sciences sociales et Sciences de l'Information Département Logique des Usages, Sciences sociales et Sciences de l'Information Département Logique des Usages, Sciences sociales et Sciences de l'Information Département Logique des Usages, Sciences sociales et Sciences de l'Information

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Résumé : One of the concerns of knowledge discovery in databases is the production of association rules. An association rule A->B defines a relationship between two sets of attributes A and B, caracterising the data studied. Such a rule means that objects sharing attributes of A will "likely" have those contained in B. Yet, this notion of "likeliness" depends on the datamining context. Many interestingness measures have been introduced in order to quantify this likeliness. This panel of measures is heterogeneous and the ranking of extracted rules, according to measures, may differ largely. This contribution explores a new approach for assessing the quality of rules: aggregating valued relations. For each measure, a valued relation is built out of the numerical values it takes on the rules, and represents the preference of a rule over another. The aim in using such tools is to take into account the intensity of preference expressed by various measures, and should reduce incomparability issues related to differences in their co-domains. It also has the advantage of relating the numerical nature of measures compared to pure binary approaches. We studied several aggregation operators. In this contribution we discuss results obtained on a toy example using the simplest of them.

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• Type de publi. : Article dans une revue
• Date de publi. : 01/04/2006
• Auteurs : Delphine DavidJean-Philippe RegnauxMichèle LejailleAlain LouisBernard BusselFrédéric Lofaso
• Organismes : Laboratoire de Synthèse Organique (Hétérochimie organique, organoéléments et matériaux) Département de neurologie Department of Neurology Physiologie et physiopathologie de la motricité chez l'homme Department of Neurological Rehabilitation
• Publié dans Archives of Physical Medicine and Rehabilitation le 27/10/2020

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Résumé : OBJECTIVE: To determine whether a gait-training (GT) machine influenced walking time duration and oxygen consumption in hemiplegic patients. DESIGN: Repeated measures with comparison of 2 groups. SETTING: Physiology laboratories in a rehabilitation hospital. PARTICIPANTS: Seven patients with stroke-related hemiplegia (2 men, 5 women; age, 46+/-11y; time since stroke, 12+/-9wk) and 7 healthy subjects (3 men, 4 women; age, 30+/-7y). INTERVENTIONS: Floor walking (FW) and GT-assisted walking with and without 50% body-weight support (BWS). MAIN OUTCOME MEASURES: Walking time duration, oxygen consumption (Vo(2)), minute ventilation (V(E)), and heart rate. RESULTS: When the condition effect was analyzed independently from the group, mean Vo(2) was higher during FW than during the GT tests (post hoc analysis: FW vs GT, P=.017; FW vs GT+BWS, P<.002). When the groups were compared independently of the condition, the group with hemiplegia had a significantly shorter walking time duration (analysis of variance [ANOVA], P<.001) and a significantly higher Vo(2) as a percentage of baseline (ANOVA, P=.03), compared with the controls. Walking time duration was influenced by walking condition (ANOVA, P<.001; post hoc analysis: FW vs GT, P<.001; FW vs GT+BWS, P<.001). Ve was influenced by walking condition (ANOVA, P=.043; not significant in the post hoc analysis) and was higher in the group with hemiplegia (ANOVA, P=.02). Heart rate was not influenced by walking condition (P=.11). A group effect was found with heart rate in cycles per minute (P=.035) but not as a percentage of baseline. No interaction was found between the ANOVA group-effect factor and the ANOVA walking-condition effect factor. CONCLUSIONS: Compared with FW, GT assistance increased walking time duration and reduced Vo(2) in patients with severe hemiplegia.

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